| Assay time | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) |
|---|---|
| Hands-on time | <1.5 hr |
| Input quantity | 1–100 ng (10 ng recommended per pool) |
| Cancer type | Solid tumor |
| Content specifications | DNA and RNA targets for 161 oncogenes |
| Description | Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers. |
| Instruments | NextSeq 550 System , NextSeq 2000 System , NextSeq 1000 System , NextSeq 550Dx in Research Mode |
| Mechanism of action | Multiplex PCR |
| Method | Targeted DNA sequencing , Amplicon sequencing , Targeted RNA sequencing |
| Multiplexing | 96 dual index combinations |
| Nucleic acid type | DNA, RNA |
| Number of amplicons | 4,648 total 4 pools (DNA pool 1: 1,891 amplicons. DNA pool 2: 1,890 amplicons. RNA pool 1: 447 amplicons. RNA pool 2: 420 amplicons.) |
| Specialized sample types | FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) |
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