20019161, AmpliSeq™ Cancer HotSpot Panel v2 for Illumina®

Overview
The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC) 1 database.

Key features
Relevant Gene Content - Target ~2800 COSMIC mutations from 50 oncogenes and tumor suppressor genes
Fast, Streamlined Workflow - Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) samples
Accurate Data - Detect somatic mutations down to 5% variant allele frequency using local or cloud-based analysis
The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Example workflow
AmpliSeq for Illumina Cancer HotSpot Panel v2
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
iSeq 100 System
MiniSeq System
MiSeq System
Local Run Manager DNA Amplicon Module
BaseSpace Sequence Hub DNA Amplicon App

Project recommendations
Instrument: Recommended number of samples
iSeq 100 System: 16 samples per run (assumes minimum coverage of 500×)
16 samples per run (assumes minimum coverage of 500×)
2 × 150 bp
MiniSeq System: Samples per run: mid output: 32, high output: 96 (assumes minimum coverage of 500×)
Samples per run: mid output: 32, high output: 96 (assumes minimum coverage of 500×)
MiSeq System: Samples per run (by reagent kit version): v2 nano: 4, v2 micro: 16, v2: 60, v3: 96 (assumes minimum coverage of 500×)
Samples per run (by reagent kit version): v2 nano: 4, v2 micro: 16, v2: 60, v3: 96 (assumes minimum coverage of 500×)

Related applications and methods
Targeted Resequencing
With targeted sequencing or resequencing, a subset of genes or a genomic region of interest is isolated and sequenced, which can conserve lab resources.
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
Cancer Genomics Research
Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.