Description
AmpliSeq for Illumina Immune Response Panel
Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.
AmpliSeq for Illumina Immune Response Panel English French Italian German Spanish Data sheet PDF < 1 MB 5 versions
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Overview
The AmpliSeq for Illumina Immune Response Panel is a targeted resequencing research assay for quantitating expression of cancer biomarkers in 395 genes involved in tumor–immune system interactions.
Panel features
Relevant Gene Content - Targeted expression of 395 cancer biomarkers indicative of immunotherapy response
Fast, Streamlined Workflow - Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality RNA or 10 ng of formalin-fixed, paraffin-embedded (FFPE) RNA input samples
Accurate Data - Detect expression of genes involved in tumor-immune interactions
The Immune Response Panel is part of an integrated RNA-to-annotated variant solution that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This panel detects biomarkers associated with different leukocyte subsets, antigen presentation, checkpoint pathways, and tumor progression. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
Example workflow
AmpliSeq for Illumina Immune Response Panel
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
NextSeq 550 System
MiSeq i100
RNA Amplicon
Local Run Manager Software
Project recommendations
Instrument: Recommended number of samples
iSeq 100 System: 4 samples per run (assumes 1 million reads per sample)
4 samples per run (assumes 1 million reads per sample)
2 × 150 bp
MiniSeq System: Samples per run: mid output: 8, high output: 24 (assumes 1 million reads per sample)
Samples per run: mid output: 8, high output: 24 (assumes 1 million reads per sample)
MiSeq System: Samples per run (by reagent kit version): v2 micro: 4, v2: 15, v3: 24 (assumes 1 million reads per sample)
Samples per run (by reagent kit version): v2 micro: 4, v2: 15, v3: 24 (assumes 1 million reads per sample)
Related applications and methods
Targeted Resequencing
With targeted sequencing or resequencing, a subset of genes or a genomic region of interest is isolated and sequenced, which can conserve lab resources.
Oncology
Illumina NGS and microarray technologies for cancer research are helping drive the revolution in cancer genomics.
Immunogenomics Research
Gain insight into the functional impact of immune-related genetic variation and potential immunological disease mechanisms with NGS and array solutions.
