20019170, AmpliSeq™ Transcriptome Human Gene Expression Panel for Illumina®

Name: AmpliSeq™ Transcriptome Human Gene Expression Panel for Illumina®
Brand: Illumina
SKU: 20019170

Sufficient for 24 reactions when paired with AmpliSeq Library PLUS for Illumina. Purchase library prep, index adapters, and accessory products separately.

Catalog Number: 20019170

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Category

NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

AmpliSeq for Illumina Transcriptome Human Gene Expression Panel
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
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Overview
The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in a single assay.

Key features
Fast gene expression profiling - Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.
Low-quality, low-quantity sample input - Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.
DNA-to-analysis solution - Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.
The Transcriptome Human Gene Expression Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Example workflow
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD indexes
NextSeq 550 System
BaseSpace Sequence Hub RNA Amplicon App
Local Run Manager RNA Amplicon Module

Related applications and methods
Targeted Resequencing
With targeted sequencing or resequencing, a subset of genes or a genomic region of interest is isolated and sequenced, which can conserve lab resources.
Cancer RNA sequencing
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
mRNA sequencing
mRNA sequencing (mRNA-Seq) using NGS can identify both known and novel transcripts, and measure transcript abundance.

Specifications

General

Assay time	6 hr (library prep only; doesn't include library quantification, normalization, or pooling time)
Hands-on time	< 1.5 hr
Input quantity	1-100 ng RNA (10 ng recommended)
Content specifications	> 95% of human RefSeq genes; 20,802 genes; captures human transcriptome
Description	Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons.
Instruments	NextSeq 550 System , NextSeq 2000 System , NextSeq 1000 System
Mechanism of action	Multiplex PCR
Method	mRNA sequencing , Targeted RNA sequencing
Multiplexing	96 dual index combinations
Nucleic acid type	RNA
Number of amplicons	1 pool
Specialized sample types	Blood, FFPE tissue
Species category	Human
Technology	Sequencing
Variant class	Transcript variants

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

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