Description
Overview
TruSeq Stranded Total RNA with Ribo-Zero Globin delivers a clear and comprehensive view of the transcriptome from blood-derived total RNA, with fast, efficient sequencing library preparation.
Key features
Uniform coverage and precise measurement of strand orientation
High-confidence discovery of alternative transcripts, antisense expression, and allele-specific expression
Discovery across both coding and multiple forms of noncoding RNA
High-quality whole-transcriptome analysis with precise strand information
These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples.
Efficient ribosomal RNA and globin mRNA removal in a single step
Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.
Traditional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.
Example workflow
TruSeq Stranded Total RNA with Ribo-Zero Globin
NextSeq 550 System
NovaSeq 6000 System
DRAGEN RNA Pipeline
BaseSpace RNA-Seq Differential Expression App
BaseSpace RNA Express App
Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
2 x 75 bp 2 x 75 bp
NovaSeq 6000 System: RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
≤ 2 × 100 bp ≤ 2 × 100 bp
Related applications and methods
Total RNA sequencing
Analyze both coding RNA and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.
Low-quality/FFPE RNA-Seq
Find FFPE RNA sequencing solutions that yield high-quality results from degraded FFPE tissues and other low-quality samples.
RNA Sequencing
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA.
Figures
Technical Replicates
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R 2 value is shown.
Even Coverage Across Transcripts
TruSeq Stranded Total RNA gives excellent coverage across the top 1,000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis = percent coverage of combined reads.
TruSeq® Stranded Total RNA Library Prep Globin (48 Samples)
20020612
Includes RiboZero beads to deplete rRNA and globin mRNA from blood samples, PCR master mix to transcript RNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters and Purification Beads separately. View less
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TruSeq® Stranded Total RNA Library Prep Globin (96 Samples)
20020613
TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)
20019792
TruSeq Stranded RNA HT Kit 96 Samples Adapter Plate Box View less
IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)
20020591
New Unique Dual Indexes (UDIs), compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA Library Prep Kits. The UDI kit is provided in a plate format, contains 24 UDIs (x4), sufficient for 96 samples. View less
IDT for Illumina – TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples)
20040871
Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022371, with no changes to the remaining 184. Purchase library preps and probe panels separately. View less
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Limited Use Label License:
The TruSeq Stranded Total RNA with Ribo-Zero Globin Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
