TruSeq RNA Exome (formerly TruSeq RNA Access Library Prep Kit)
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
TruSeq RNA Exome Data Sheet English French Italian German Spanish Data sheet PDF < 1 MB 5 versions
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The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.
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Overview
TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.
Key features
Affordability and focus - Isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
High-quality data from difficult samples - Optimized for sequencing RNA from degraded samples, including FFPE tissues
Samples with limited starting material - Greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity
TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.
Example workflow
mRNA sequencing
NextSeq 550 System
NovaSeq 6000 System
DRAGEN RNA pipeline
RNA-seq differential expression
BaseSpace RNA Express App
Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: 5 to 16 samples per run (based on 25 million reads per sample)
5 to 16 samples per run (based on 25 million reads per sample)
2 x 75 bp
NovaSeq 6000 System: Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations)
Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations)
2 × 100 bp
Related applications and methods
Low-Quality/FFPE RNA-Seq Solutions
Find FFPE RNA sequencing solutions that yield high-quality results from degraded FFPE tissues and other low-quality samples.
Cancer RNA sequencing
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
How others use this product
Looking beyond single-drug approaches to precision oncology research
A tale of two RNA library prep kits
Figures
Efficient Gene Fusion Discovery
TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.
Resources
Ebola and sexual transmission
Six months after being discharged from the Ebola treatment, a male survivor of Ebola Virus Disease (EVD) transmitted the virus to his female partner, who died of the disease. The survivor’s semen had tested negative by conventional means, but the authors were able to enrich the semen sample for EBOV RNA with the Illumina TruSeq RNA Access Kit and custom probes.
TruSeq® RNA Library Prep for Enrichment (48 Samples)
20020189
Includes reagents for preparing 48 libraries. Purchase index adapters, enrichment panel, and enrichment reagents separately. View less
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TruSeq® RNA Enrichment (12 enrichments)
20020490
Sufficient for 12 enrichment reactions when paired with TruSeq RNA Library Prep for Enrichment. Purchase library prep, index adapters, and probe panels separately. View less
TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)
20020492
Includes 12 of 24 indexes (Indexes: 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, 19) for preparing 48 individual RNA samples. View less
TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)
20020493
Includes 12 of 24 indexes (Indexes: 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27) for preparing 48 individual RNA samples. View less
Illumina Exome Panel – Enrichment Oligos Only
20020183
Sufficient for 8 enrichment reactions when paired with Illumina DNA Prep with Enrichment, 12 reactions when paired with TruSeq RNA Exome, and 32 reactions when paired with Illumina RNA prep with enrichment. Purchase library prep and enrichment reagents and index adapters separately. View less
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