Overview
TruSeq Stranded mRNA offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It is compatible with a wide range of samples.
Strand orientation detection
Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.
Cost-efficient
Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.
Scalable
The workflow enables robust interrogation of both standard and low-quality samples, and workflows compatible with a wide range of study designs.
Fast, automation-friendly workflow
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners .
Example workflow
TruSeq Stranded mRNA
NextSeq 550Dx Instrument
NovaSeq 6000 System
DRAGEN RNA Pipeline
RNA-Seq Differential Expression
BaseSpace RNA Express App
Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: RNA Profiling: 13–40 samples per run (based on 10 million reads per sample) Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
RNA Profiling: 13–40 samples per run (based on 10 million reads per sample) Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
2 x 75 bp 2 x 75 bp
NovaSeq 6000 System: RNA Profiling (samples per run, dual flow cell): S1: 320, S2:384, S4:768. Limited by index combinations (dual). Based on 10 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 128, S2: 256, S4: 768. Limited by index combinations (dual). Based on 25 million reads.
RNA Profiling (samples per run, dual flow cell): S1: 320, S2:384, S4:768. Limited by index combinations (dual). Based on 10 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 128, S2: 256, S4: 768. Limited by index combinations (dual). Based on 25 million reads.
≤ 2 × 100 bp ≤ 2 × 100 bp
Related applications and methods
mRNA sequencing
mRNA sequencing (mRNA-Seq) using NGS can identify both known and novel transcripts, and measure transcript abundance.
Cancer RNA sequencing
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
Gene expression analysis
Learn how to profile gene expression changes for a deeper understanding of biology.
How others use this product
Researchers at the Icahn School of Medicine at Mount Sinai are studying mtDNA, piRNA, and TCR repertoire diversity to unlock the secrets of cancer, infertility, and autoimmune disease.
RNA-Seq and HLA typing are increasing the power and efficiency of a target discovery platform.
Figures
Technical Replicates
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R 2 value is shown.
TruSeq® Stranded mRNA Library Prep (48 Samples)
20020594
Includes RNA purification beads for upfront polyA capture step, PCR master mix to transcript mRNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters separately. Purfication beads are required post polyA capture step and are sold separately. View less
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TruSeq Stranded mRNA Library Prep (96 Samples)
20020595
TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)
20020492
Includes 12 of 24 indexes (Indexes: 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, 19) for preparing 48 individual RNA samples. View less
TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)
20020493
Includes 12 of 24 indexes (Indexes: 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27) for preparing 48 individual RNA samples. View less
IDT for Illumina – TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples)
20040871
Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022371, with no changes to the remaining 184. Purchase library preps and probe panels separately. View less
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This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
