TruSeq Stranded Total RNA | Analyze coding and non-coding RNA
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
TruSeq Stranded mRNA and Total RNA English French Italian German Spanish Data sheet PDF 8 MB 5 versions
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The products previously known as TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Cat. No. RS-122-2201, RS-122-2202, and RS-122-2203) and TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Cat. No. RS-122-2301, RS-122-2302, and RS-122-2303) are now called TruSeq Stranded Total RNA Human/Mouse/Rat and TruSeq Stranded Total RNA Gold, respectively. The product configuration has changed. You'll need to purchase the library prep and index adapter products separately (instead of in a kit).
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Overview
TruSeq Stranded Total RNA provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.
Whole-Transcriptome analysis with precise measurement of strand orientation
Compatible with a wide range of samples, including low-quality DNA/RNA and formalin-fixed, paraffin-embedded (FFPE) samples, TruSeq Stranded Total RNA couples all of the benefits of TruSeq RNA technology with Ribo-Zero ribosomal RNA reduction chemistry. The product enables analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.
Species compatibility
TruSeq Stranded Total RNA is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.
Flexible solutions for a range of study designs
TruSeq Stranded Total RNA is available in multiple versions to fit specific study design needs:
TruSeq Stranded Total RNA Human/Mouse/Rat removes cytoplasmic rRNA
TruSeq Stranded Total RNA Gold removes both cytoplasmic and mitochondrial rRNA
The total RNA kits are also compatible with the new unique dual (UD) index kits, IDT for Illumina - TruSeq RNA UD Indexes , which allows multiplexing of up to 96 samples
Fast, automation-friendly workflow
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners .
Example workflow
TruSeq Stranded Total RNA
NextSeq 550 System
NovaSeq 6000 System
DRAGEN RNA
BaseSpace RNA-Seq Differential Expression
BaseSpace RNA Express App
Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: RNA profiling: 6-20 samples per run (based on 20 million reads per sample) Transcriptome analysis: 2-8 samples per run (based on 50 million reads per sample)
RNA profiling: 6-20 samples per run (based on 20 million reads per sample) Transcriptome analysis: 2-8 samples per run (based on 50 million reads per sample)
2 x 75 bp
NovaSeq 6000 System: RNA profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
RNA profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
≤ 2 × 100 bp
Related applications and methods
Total RNA sequencing
Analyze both coding RNA and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.
Low-quality/FFPE RNA-Seq
Find FFPE RNA sequencing solutions that yield high-quality results from degraded FFPE tissues and other low-quality samples.
RNA sequencing
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA.
High quality strand information
Consistent, precise measurement of RNA abundance is reflected by high reproducibility between technical replicates. These technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R 2 value is shown.
Even coverage across transcripts
TruSeq Stranded Total RNA gives excellent coverage across the top 1000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis: percent coverge of combined reads.
TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (48 Samples)
20020596
Includes RiboZero beads to deplete rRNA from human, mouse, and rat, PCR master mix to transcript RNA into cDNA , and reagents for preparing 48 samples. Purchase index adapters and Purification Beads separately. View less
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TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)
20020597
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
20020598
Includes RiboZero beads to deplete rRNA and mitochondrial rRNA from human, mouse, and rat, PCR master mix to transcript RNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters separately. Purchase index adapters and Purification Beads separately. View less
TruSeq® Stranded Total RNA Library Prep Gold (96 Samples)
20020599
TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)
20020492
Includes 12 of 24 indexes (Indexes: 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, 19) for preparing 48 individual RNA samples. View less
TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)
20020493
Includes 12 of 24 indexes (Indexes: 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27) for preparing 48 individual RNA samples. View less
IDT for Illumina – TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples)
20040871
Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022371, with no changes to the remaining 184. Purchase library preps and probe panels separately. View less
Illumina Ribo-Zero plus rRNA Depletion Kit (96 Samples)
20037135
Includes Ribo-Zero Plus depletion module for depletion of ribosomal RNA from human, mouse, rat, and bacteria and globin transcripts for 96 samples. Purchase purification beads, library prep reagents and index adapters separately. For complete kit, including library prep reagents, order the Illumina Stranded Total RNA with Ribo-Zero Plus kit. View less
TruSeq Total RNA Training
15044069
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Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
