Illumina, 20020611, TruSeq® Stranded Total RNA Library Prep Plant (96 Samples)

High-quality whole-transcriptome analysis with precise strand information
RNA-Seq technology provides uniform coverage, precise measurement of strand orientation, and high-confidence discovery of features such as alternative transcripts, antisense expression, and allele-specific expression across both coding and multiple forms of noncoding RNA. TruSeq Stranded Total RNA with Ribo-Zero Plant couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Efficient ribosomal RNA reduction across a range of plant species, study designs
TruSeq Stranded Total RNA with Ribo-Zero Plant enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. These products have been validated for use in multiple plant species, including Arabidopsis thaliana , rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.

Example workflow
Total RNA sequencing
NextSeq 550 System
NovaSeq 6000 System
DRAGEN RNA Pipeline
BaseSpace RNA-Seq Differential Expression App
BaseSpace RNA Express App

Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
2 x 75 bp 2 x 75 bp
NovaSeq 6000 System: RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
≤ 2 × 100 bp ≤ 2 × 100 bp

Related applications and methods
Plant and animal sequencing
RNA sequencing
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA.
Total RNA sequencing
Analyze both coding RNA and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.