20023378, AmpliSeq™ for Illumina® Direct FFPE DNA

Name: AmpliSeq™ for Illumina® Direct FFPE DNA
Brand: Illumina
SKU: 20023378

Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.

Catalog Number: 20023378

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Category

NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

Overview
The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes.

Key features
Relevant gene content - Target all exonic regions and flanking intronic sequences of BRCA1 and BRCA2
Fast, streamlined workflow - Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate data - Detect somatic mutations as low as 5% variant allele frequency using local or cloud based analysis
The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels.

Example workflow
AmpliSeq for Illumina BRCA Panel
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
iSeq 100 System
MiniSeq System
MiSeq System
BaseSpace Sequence Hub DNA Amplicon App
Local Run Manager DNA Amplicon Module

Project recommendations
Instrument: Recommended number of samples
iSeq 100 System: 12 samples per run (assumes minimum coverage of 500×) 96 samples per run (assumes minimum coverage of 50x)
12 samples per run (assumes minimum coverage of 500×) 96 samples per run (assumes minimum coverage of 50x)
2 × 150 bp 2 × 150 bp
MiniSeq System: Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×)
Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×)
2 × 150 bp
MiSeq System: Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80 Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96
Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80 Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96

Related applications and methods
Targeted resequencing
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.

Specifications

General

Assay time	5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Hands-on time	<1.5 hr
Input quantity	1–100 ng (10 ng recommended per pool)
Cancer type	Solid tumor
Content specifications	Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2
Description	Germline and somatic analysis studies of BRCA1 and BRCA2 .
Instruments	MiSeq System , iSeq 100 System , MiSeqDx in Research Mode , MiniSeq System
Mechanism of action	Multiplex PCR
Method	Targeted DNA sequencing , Amplicon sequencing
Multiplexing	96 dual index combinations
Nucleic acid type	DNA
Number of amplicons	265 total. 2 pools. (Pool 1: 132 amplicons. Pool 2: 133 amplicons).
Specialized sample types	Blood, FFPE tissue
Species category	Human
Technology	Sequencing
Variant class	Single nucleotide polymorphisms (SNPs), Somatic variants, Germline variants, Insertions-deletions (indels)

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

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