20023393, AmpliSeq™ for Illumina® RNA Library Preparation Kit Training

Name: AmpliSeq™ for Illumina® RNA Library Preparation Kit Training
Brand: Illumina
SKU: 20023393

1.5 day on-site training for the complete AmpliSeq™ for Illumina RNA library preparation workflow. This training can be delivered with any of the available AmpliSeq™ for Illumina RNA panels. Includes quantification of RNA input as well as best practices, troubleshooting tips, and training on Illumina-supported analysis tools specific to the RNA application workflow.

Catalog Number: 20023393

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NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

AmpliSeq for Illumina Custom RNA Panel
Targeted, custom RNA research panels optimized for sequencing up to 1200 genomic targets of interest.
AmpliSeq for Illumina Custom RNA Sequencing Panel Data sheet HTML externalFile
Data sheet HTML externalFile
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Overview
Use AmpliSeq for Illumina Custom RNA Panels to measure gene expression in 12 to 1200 gene targets in a single assay. You can easily design custom targeted panels from a menu of over 20,000 human RefSeq genes using DesignStudio Software, a web-based assay design software tool.

Key features
Fast Gene Expression Profiling - Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.
Low-Quality, Low-Quantity Sample Input - Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.
DNA-to-Analysis Solution - Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.
An AmpliSeq for Illumina Custom RNA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
Learn More About Ampliseq for Illumina

Example workflow
DesignStudio Software
AmpliSeq for Illumina Library Prep PLUS
AmpliSeq for Illumina CD Indexes
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq System
BaseSpace Sequence Hub RNA Amplicon App

Related applications and methods
Targeted Resequencing
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Amplicon Sequencing
Ultra-deep sequencing of PCR amplicons enables analysis of specific genomic regions of interest. Learn more about amplicon sequencing and find comprehensive solutions.

Figures
High Concordance between Replicates
Libraries were prepared from 10 ng of Universal Human RNA using an AmpliSeq for Illumina Custom RNA Panel (208 amplicon panel size) and sequenced on the MiSeq System. Results show a high concordance (R2 = 0.997) between the two replicates of the same RNA sample. R2 is a statistical measurement of data correlation.
High Read Alignment
The AmpliSeq for Illumina Custom RNA Panel enables researches to analyze differential gene expression across genes of interest. To demonstrate assay performance, Human Brain Reference RNA and Universal Human RNA were analyzed using 5 AmpliSeq for Illumina Custom RNA panels of varying size (number of amplicons). Libraries were sequenced on a MiSeq System.
AmpliSeq™ for Illumina® Custom RNA Panel
20020496
Targeted custom RNA panel. Library PLUS and index adapters sold separately. View less
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set B for Illumina®
20019106
AmpliSeq™ CD Indexes Set C for Illumina®
20019107
AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately. View less
AmpliSeq™ for Illumina® RNA Library Preparation Kit Training
20023393
1.5 day on-site training for the complete AmpliSeq™ for Illumina RNA library preparation workflow. This training can be delivered with any of the available AmpliSeq™ for Illumina RNA panels. Includes quantification of RNA input as well as best practices, troubleshooting tips, and training on Illumina-supported analysis tools specific to the RNA application workflow. View less
Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels). View less
AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing. View less
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Specifications

General

Assay time	5.5-7.5 hours
Hands-on time	< 1.5 hr
Input quantity	1 ng
Instruments	MiSeq System , iSeq 100 System , NextSeq 550 System , NextSeq 2000 System , NextSeq 1000 System , MiniSeq System , NextSeq 500 System
Mechanism of action	Multiplex PCR
Method	Custom sequencing , Amplicon sequencing , Targeted RNA sequencing
Multiplexing	Up to 384-plex
Nucleic acid type	RNA
Number of amplicons	Varies
Specialized sample types	Blood, FFPE tissue
Species category	Human
Technology	Sequencing
Variant class	Transcript variants

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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