Overview
Illumina DNA Prep with Enrichment, previously known as Nextera Flex for Enrichment, is the fastest and most flexible targeted sequencing solution for DNA in the Illumina library prep portfolio.
Library preparation Complete library prep, enrichment, and post-enrichment amplification in only 6.5 hr. Get consistent library yields with highly uniform insert sizes. Enhance library preparation efficiency with integrated protocols for blood and saliva.
Library preparation
Complete library prep, enrichment, and post-enrichment amplification in only 6.5 hr. Get consistent library yields with highly uniform insert sizes. Enhance library preparation efficiency with integrated protocols for blood and saliva.
Fast, user-friendly workflow With built-in quantification and library normalization, on-bead tagmentation chemistry combined with a simplified, single hybridization protocol reduces hands-on time to ~2 hr.
Fast, user-friendly workflow
With built-in quantification and library normalization, on-bead tagmentation chemistry combined with a simplified, single hybridization protocol reduces hands-on time to ~2 hr.
Sample input flexibility Compatible with a broad range of DNA inputs and sample types, including blood, saliva, genomic DNA, and formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample input flexibility
Compatible with a broad range of DNA inputs and sample types, including blood, saliva, genomic DNA, and formalin-fixed, paraffin-embedded (FFPE) tissue.
Optimized performance On-bead tagmentation minimizes bias and opportunities for error, resulting in highly reproducible data across all Illumina sequencing systems.
Optimized performance
On-bead tagmentation minimizes bias and opportunities for error, resulting in highly reproducible data across all Illumina sequencing systems.
Flexible Content Options Source your enrichment panel from Illumina, with options for pre-designed panels with fixed content to customized panels designed from scratch for your target list of interest. Looking for a whole exome sequencing solution? See our complete library prep kit .
Flexible Content Options
Source your enrichment panel from Illumina, with options for pre-designed panels with fixed content to customized panels designed from scratch for your target list of interest.
Looking for a whole exome sequencing solution? See our complete library prep kit .
Illumina DNA Prep product line
Find fast, optimized sequencing library preparation solutions for use in a wide range of applications.
Illumina DNA Prep with Enrichment
Fast, integrated DNA-based library preparation and enrichment workflow combining on-bead tagmentation with a single hybridization protocol for targeted sequencing applications.
Illumina DNA Prep
Fast, integrated library preparation workflow for a wide range of applications, including sequencing of whole human genomes, amplicons, plasmids, and microbial genomes.
Illumina DNA PCR-Free Prep
High-performing, fast, and integrated PCR-free library preparation workflow for sensitive applications such as human whole-genome sequencing or tumor–normal sequencing.
Applications
The Illumina DNA Prep with Enrichment combines versatile, simple, and fast library preparation and enrichment from a wide range of DNA inputs and sample types to support broad target enrichment applications encompassing custom panels, fixed panels, and whole-exome panels.
Example workflow
Illumina DNA Prep with Enrichment
Illumina DNA Prep with Exome 2.5 Enrichment
Illumina Custom Enrichment Panel v2
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq 550 System
NextSeq 1000 and 2000 Systems
NovaSeq 6000 System
NovaSeq X Series
DRAGEN Enrichment
DRAGEN Germline Pipeline
DRAGEN Somatic Pipeline
Project recommendations
Instrument: Recommended number of samples
iSeq 100 System: TruSight Cancer: 4 samples per run (based on 280× mean targeted coverage; 99% target coverage at 20×)
TruSight Cancer: 4 samples per run (based on 280× mean targeted coverage; 99% target coverage at 20×)
2 x 100 bp
MiniSeq System: TruSight Cancer (samples per run): mid output: 8, high output: 25 (based on 280× mean targeted coverage; 99% target coverage at 20×)
TruSight Cancer (samples per run): mid output: 8, high output: 25 (based on 280× mean targeted coverage; 99% target coverage at 20×)
MiSeq System: TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 280× mean targeted coverage; 99% target coverage at 20×) TruSight One (samples per run): v2 reagents: 1, v3 reagents: 3 (based on 120× mean targeted coverage; 95% target coverage at 20×)
TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 280× mean targeted coverage; 99% target coverage at 20×) TruSight One (samples per run): v2 reagents: 1, v3 reagents: 3 (based on 120× mean targeted coverage; 95% target coverage at 20×)
2 x 100 bp 2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible)
NextSeq 550 System: TruSight One (samples per run): mid output: 12, high output: 36 (based on 120× mean targeted coverage; 95% target coverage at 20×) TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130× mean targeted coverage; 97% target coverage at 20×)
TruSight One (samples per run): mid output: 12, high output: 36 (based on 120× mean targeted coverage; 95% target coverage at 20×) TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130× mean targeted coverage; 97% target coverage at 20×)
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp (up to 2 x 150 bp is possible)
NovaSeq 6000 System: Exome sampels per run, by flow cell type) - Illumina DNA Prep with Exome 2.5 Enrichment : SP: 34, S1: 69, S2: 176, S4: 428 based on 50× mean target coverage, 42M reads, 4.2 Gb of data. Note: S4: 428 requires NovaSeq Xp workflow or additional indexes.
Exome sampels per run, by flow cell type) - Illumina DNA Prep with Exome 2.5 Enrichment : SP: 34, S1: 69, S2: 176, S4: 428 based on 50× mean target coverage, 42M reads, 4.2 Gb of data. Note: S4: 428 requires NovaSeq Xp workflow or additional indexes.
Related applications and methods
Targeted resequencing
With targeted sequencing or resequencing, a subset of genes or a genomic region of interest is isolated and sequenced, which can conserve lab resources.
Cancer genomics research
Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.
Complex and genetic disease research
Comprehensive array and next-generation sequencing solutions to accelerate research of various genetic complex diseases.
Illumina on-bead tagmentation chemistry
A uniform tagmentation reaction mediated by eBLTs followed by a single hybridization reaction enables a fast and flexible workflow.
Enrichment panel compatibility
Illumina DNA Prep with Enrichment is compatible with various Illumina enrichment panels and is optimized for use on low-, mid-, and high-throughput systems.
High coverage uniformity and padded read enrichment
Illumina DNA Prep with Enrichment provides high coverage uniformity and on-target padded read enrichment for custom, fixed, and exome panels.
Accurate variant calling
Illumina DNA Prep with Enrichment provides low-abundance somatic variant calling for (A, B) cell line FFPE control human reference DNA samples and (C, D) real-world FFPE samples with observed variant frequency showing significant correlation with frequencies from an orthologous sequencing assay.
Resources
Illumina DNA Prep with Enrichment: Introduction
Optimized workflows for custom targeted enrichment, from oligo probe design to NGS data analysis
Comprehensive solution, from probe design to data analysis, for multiple targeted sequencing applications. Our custom-designed panels ensure high uniformity and coverage, with enhanced coverage of GC-rich and difficult to sequence areas.
Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)
20025524
Includes reagents for preparing and enriching 96 libraries (eight, 12-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. View less
Sign in to add to cart or see pricing.
List Price:
Discounts:
Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)
20025523
Includes reagents for preparing and enriching 16 libraries (16, 1-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. View less
Illumina® DNA Prep, (S) Tagmentation (96 Samples)
20025520
Includes reagents for preparing 96 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (96 samples)). View less
Illumina® DNA Prep, (S) Tagmentation (16 Samples)
20025519
Includes reagents for preparing 16 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (16 samples)). View less
TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions)
20029551
TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions) View less
TruSight One – Enrichment Oligos only (6 Enrichment Reactions)
20029227
Sufficient for six enrichment reactions when paired with Illumina DNA Prep with Enrichment, (S) Tagmentation. Purchase library prep and enrichment reagents and index adapters separately. View less
TruSight One Expanded – Enrichment Oligos only (6 Enrichment Reactions)
20029226
Illumina Custom Enrichment Panel V2 (32 µl, 120 bp)
20073953
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 32 µl of material (sufficient for 8 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately. View less
Illumina Custom Enrichment Panel V2 (384 µl, 120 bp)
20073952
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 384 µl of material (sufficient for 96 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately. View less
Illumina Custom Enrichment Panel V2 (1536 µl, 120 bp)
20111339
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 1536 µl of material (sufficient for 384 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately. View less
Illumina Custom Enrichment Panel (8 Enrichment Reactions)
20025371
Sufficient for eight enrichment reactions when paired with Illumina DNA Prep with Enrichment. Enables custom panel design between 2K–67K probes using DesignStudio. Purchase library prep and enrichment reagents and index adapters separately. View less
Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)
20091654
Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)
20091656
Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)
20091658
Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)
20091660
Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
Illumina DNA Prep Enrichment-Customer Site
20028457
A 1.5-day onsite training for the complete Illumina DNA Prep Enrichment library preparation workflow, with detailed hands-on instruction. This training can be delivered with select Illumina fixed panels (Exome or TruSight Cancer), Illumina custom DNA panels, or select third party DNA panels (IDT xGen Exome or Twist Human Core Exome). Participants will learn all steps of the workflow including sample input and library preparation, enrichment, quality control, best practices, and troubleshooting, in addition to training on Illumina-supported sequencing and data analysis tools specific to the Illumina DNA Prep with Enrichment workflow. View less
Illumina Purification Bead, 100mL
20060057
Includes one, 100-ml container of beads for library clean-up and size selection. Purchase library prep and enrichment reagents, probe panels, and indexes separately. View less
Illumina Purification Bead, 400mL
20060058
Includes four, 100-ml container of beads for library clean-up and size selection. Purchase library prep and enrichment reagents, probe panels, and indexes separately. View less
Flex Lysis Reagent Kit (96 reactions)
20018706
Includes reagents for processing blood samples with the Illumina DNA Prep and Illumina DNA Prep with Enrichment. Purchase library prep, enrichment, enrichment probe panel, and index adapter reagents separately. View less
Infinium FFPE QC Kit (384 reactions)
WG-321-1001
Infinium FFPE QC Kit is an accessory product required to qualify FFPE samples with Nextera Flex for Enrichment. Library prep, enrichment, enrichment probe panel, and index adapter reagents need to be ordered separately. View less
Infinium MIDI Heatblock Insert
BD-60-601
Heatblock insert for the SciGene Hybex Microheating system required for the Nextera Flex for Enrichment hybridization steps. View less
Illumina® Free Adapter Blocking Reagent (12 Reactions)
20024144
This reagent is used during library preparation to reduce index hopping levels. View less
Illumina® Free Adapter Blocking Reagent (48 Reactions)
20024145
Showing of
Product
Qty
Unit price
Remove
Total ( items)
You have not selected any items yet.
Please add these products to your cart before leaving the page to avoid losing them.
How much DNA do I need?
The Illumina DNA Prep with Enrichment kit is compatible with high-quality, double-stranded genomic DNA (gDNA) inputs of 10–1000 ng or 50-1000 ng FFPE. For human gDNA samples and other large complex genomes, the recommended minimum gDNA input is 50 ng.
How does the Illumina DNA Prep and Enrichment work?
The kit uses a bead-based transposome complex to fragment and tag genomic DNA with adaptors followed by a PCR step that adds indexed adapter sequences to the ends of a DNA fragment. After library prep, targets are enriched by hybridizing biotinylated oligonucleotide probes and capturing the targeted library fragments using Streptavidin Magnetic Beads (SMB). The captured, indexed libraries are eluted from the beads and amplified before sequencing.
Can frozen or clotted blood be used with the Flex Lysis Reagent Kit and Blood Lysis protocol for Illumina DNA Prep with Enrichment?
Use of frozen or clotted blood with the Flex Lysis Reagent Kit and Blood Lysis protocol with Illumina DNA Prep with Enrichment is not supported or tested by Illumina.
What blood tubes have been tested with Illumina DNA Prep with Enrichment?
The Blood Lysis protocol with Illumina DNA Prep with Enrichment is optimized for the use with K2-EDTA collection tubes (manufactured by Becton Dickinson). Other blood collection tubes may work, but they have not been validated.
Frequently purchased
Other products to consider
