20024183, Ampliseq™ for Illumina® Focus Panel MiSeq Kit

Name: Ampliseq™ for Illumina® Focus Panel MiSeq Kit
Brand: Illumina
SKU: 20024183

The Ampliseq™ Focus Panel MiSeq Kit contains library prep and MiSeq sequencing reagents for 48 samples. This includes two AmpliSeq for Illumina® Focus Panels, one Ampliseq™ for Illumina® Library PLUS (96 Reactions), one Ampliseq™ for Illumina® CD Indexes Set A, and two MiSeq Reagent Kits v2 300 cycle.

Catalog Number: 20024183

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NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

Overview
The AmpliSeq for Illumina Focus Panel is a targeted resequencing assay for biomarker analysis of 52 genes with known relevance to solid tumors. Using the Focus Panel, researchers can analyze both DNA and RNA concurrently.

Key features
Relevant gene content - Target biomarkers across 52 genes relevant to solid tumors
Fast, streamlined workflow - Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate data - Detect somatic mutations down to 5% frequency using local or cloud-based analysis
The Focus Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Example workflow
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
AmpliSeq for Illumina Focus Panel
iSeq 100 System
MiniSeq System
MiSeq System
BaseSpace Sequence Hub DNA Amplicon App
BaseSpace Sequence Hub RNA Amplicon App
BaseSpace Sequence Hub Lab CNV App
Local Run Manager DNA Amplicon Module
Local Run Manager RNA Amplicon Module

Project recommendations
Instrument: Recommended number of samples
iSeq 100 System: 8 samples per run (assumes minimum coverage of 500×)
8 samples per run (assumes minimum coverage of 500×)
2 × 150 bp
MiniSeq System: Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×)
Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×)
MiSeq System: Samples per run (by reagent kit version): v2 micro: 8, v2: 30, v3: 48 (assumes minimum coverage of 500×)
Samples per run (by reagent kit version): v2 micro: 8, v2: 30, v3: 48 (assumes minimum coverage of 500×)

Related applications and methods
Targeted resequencing
With targeted sequencing or resequencing, a subset of genes or a genomic region of interest is isolated and sequenced, which can conserve lab resources.
Library prep and array kit selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.

Figures
High coverage uniformity and on-target alignment
DNA extracted from FFPE tissues of varying quality and HD samples was evaluated using the AmpliSeq for Illumina Focus Panel and sequenced on the MiniSeq and MiSeq Systems. Cq is an indicator of the quality of DNA islolated from FFPE tissues.
High concordance between expected and detected variant frequency
DNA from formalin-fixed HD samples was evaluated using the AmpliSeq for Illumina Focus Panel and sequenced on the MiniSeq and MiSeq Systems. Results showed 100% concordance between the expected detected variant frequency.
AmpliSeq™ Focus Panel for Illumina®
20019164
Focused DNA and RNA targeted panel that targets SNVs, Indels, CNVs and gene fusions. Kit contains 24 reactions. Library PLUS and index adapters sold separately. View less
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Ampliseq™ for Illumina® Focus Panel MiSeq Kit
20024183
The Ampliseq™ Focus Panel MiSeq Kit contains library prep and MiSeq sequencing reagents for 48 samples. This includes two AmpliSeq for Illumina® Focus Panels, one Ampliseq™ for Illumina® Library PLUS (96 Reactions), one Ampliseq™ for Illumina® CD Indexes Set A, and two MiSeq Reagent Kits v2 300 cycle. View less
AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set B for Illumina®
20019106
AmpliSeq™ CD Indexes Set C for Illumina®
20019107
AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately. View less
Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels). View less
AmpliSeq™ for Illumina® Sample ID Panel
20019162
Includes eight single nucleotide polymorphism (SNP)-targeting primer pairs and one gender discriminating primer pair sufficient for 96 reactions when paired with AmpliSeq Library PLUS for Illumina. Enables quick and accurate sample identification. Purchase library prep, probe panels, and index adapters separately. View less
AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification. View less
AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing. View less
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Specifications

General

Assay time	5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time)
Hands-on time	< 1.5 hr
Input quantity	1–100 ng (10 ng recommended per pool)
Cancer type	Solid tumor
Content specifications	DNA and RNA targets for 52 oncogenes
Description	Somatic analysis research on 52 genes associated with solid tumor cancers.
Instruments	MiSeq System , iSeq 100 System , MiSeqDx in Research Mode , MiniSeq System
Mechanism of action	Multiplex PCR
Method	Targeted DNA sequencing , Amplicon sequencing , Targeted RNA sequencing
Multiplexing	96 dual index combinations
Nucleic acid type	DNA, RNA
Number of amplicons	553 total. 2 pools. (DNA Pool: 269 amplicons. RNA Pool: 284 amplicons.)
Specialized sample types	FFPE tissue
Species category	Human
Technology	Sequencing
Variant class	Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs)

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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