20024478, AmpliSeq™ for Illumina® Myeloid Panel

Name: AmpliSeq™ for Illumina® Myeloid Panel
Brand: Illumina
SKU: 20024478

Fixed panel includes 74 genes associated with hematologic malignancies. Kit contains 24 reactions (24 DNA libraries and 24 RNA libraries). Requires 48 reactions of LP PLUS. Library PLUS, indexes and cDNA synthesis sold separately.

Catalog Number: 20024478

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Category

NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

Overview
The Myeloid Panel enables concurrent analysis of both DNA and RNA from blood and bone marrow samples in a single assay to study biomarkers associated with hematologic malignancies. The panel covers relevant targets for these major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML). Key features include:

Key features
High-value content - Targets biomarkers across 69 genes including challenging targets such as CEBPA and FLT3-ITDs
Fast, streamlined workflow - Prepare sequencing-ready libraries in a single day from as little as 10 ng high-quality DNA and RNA
Accurate data - Detect somatic mutations down to 5% frequency using local or cloud-based analysis
The Myeloid Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis.
The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Example workflow
AmpliSeq for Illumina Myeloid Panel
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
MiniSeq system
MiSeq system
BaseSpace Sequence Hub DNA amplicon app
BaseSpace Sequence Hub RNA amplicon app
Local Run Manager RNA amplicon module

Project recommendations
Instrument: Recommended number of samples
MiniSeq System: Samples per run (mid output): 4 DNA, 32 RNA, 3 combined samples (based on >95% at 500x minimum coverage) Samples per run (high output): 12 DNA, 96 RNA, 11 combined samples (based on >95% at 500x minimum coverage)
Samples per run (mid output): 4 DNA, 32 RNA, 3 combined samples (based on >95% at 500x minimum coverage) Samples per run (high output): 12 DNA, 96 RNA, 11 combined samples (based on >95% at 500x minimum coverage)
2 x 151 bp 2 x 151 bp
MiSeq System: Samples per run with v2 reagents: 7 DNA, 60 RNA, 6 combined samples Samples per run with v3 reagents: 12 DNA, 96 RNA, 11 combined samples
Samples per run with v2 reagents: 7 DNA, 60 RNA, 6 combined samples Samples per run with v3 reagents: 12 DNA, 96 RNA, 11 combined samples

Related applications and methods
Targeted resequencing
With targeted sequencing or resequencing, a subset of genes or a genomic region of interest is isolated and sequenced, which can conserve lab resources.
Oncology
Illumina NGS and microarray technologies for cancer research are helping drive the revolution in cancer genomics.

Figures
High coverage of CEBPA gene
DNA evaluated with AmpliSeq for Illumina Myeloid Panel shows high read depth coverage across the entire transcript of the gene.
AmpliSeq™ for Illumina® Myeloid Panel
20024478
Fixed panel includes 74 genes associated with hematologic malignancies. Kit contains 24 reactions (24 DNA libraries and 24 RNA libraries). Requires 48 reactions of LP PLUS. Library PLUS, indexes and cDNA synthesis sold separately. View less
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List Price:
Discounts:
AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set B for Illumina®
20019106
AmpliSeq™ CD Indexes Set C for Illumina®
20019107
AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately. View less
Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels). View less
AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing. View less
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Specifications

General

Assay time	5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Hands-on time	< 1.5 hr
Input quantity	20 ng high-quality DNA; 10 ng high-quality RNA (10 ng recommended per pool)
Cancer type	Hematologic
Content specifications	Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels)
Description	Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers.
Instruments	MiSeq System , MiniSeq System
Method	Targeted DNA sequencing , Amplicon sequencing , Targeted RNA sequencing
Multiplexing	Up to 96-plex
Nucleic acid type	DNA, RNA
Specialized sample types	Blood, Not FFPE-compatible, Bone marrow
Species category	Human
Technology	Sequencing
Variant class	Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels)

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

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