20024479, AmpliSeq™ for Illumina® Immune Repertoire Plus, TCR beta Panel

Name: AmpliSeq™ for Illumina® Immune Repertoire Plus, TCR beta Panel
Brand: Illumina
SKU: 20024479

Sufficient for 24 reactions when paired with AmpliSeq Library PLUS for Illumina. Purchase library prep, index adapters, and accessory products separately.

Catalog Number: 20024479

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NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel
Targeted RNA research panel to investigate T cell diversity and clonal expansion by sequencing T cell receptor beta chain rearrangements.
AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel Data Sheet Data sheet HTML externalFile
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Hands-on time
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Overview
AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel is a highly multiplexed targeted resequencing panel to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. Use a single pool of multiplex PCR primers, library reagents, and sample barcodes, to generate libraries from RNA extracted from whole blood, fresh-frozen tissue, or FACS-sorted cells.

Key features
High-value content - Obtain comprehensive coverage of TCR beta chain sequence
Fast, streamlined workflow - Prepare sequencing-ready libraries in a single day from whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS) cells
Low input requirement - Generate high-quality sequencing libraries from as little as 10 ng of input RNA
The Immune Repertoire Plus, TCR beta Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Example workflow
AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
AmpliSeq for Illumina CDNA Synthesis
MiniSeq System
MiSeq System
BaseSpace Sequence Hub RNA Amplicon App
Local Run Manager RNA Amplicon Module

Project recommendations
Instrument: Recommended number of samples
MiniSeq System: Samples per run will depend on sample type.
Samples per run will depend on sample type.
2 × 150 bp
MiSeq System: Samples per run will depend on sample type.
2 x 300 bp
NextSeq 550 System: Samples per run will depend on sample type.

Related applications and methods
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Immunogenomics Research
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Figures
AmpliSeq™ for Illumina® Immune Repertoire Plus, TCR beta Panel
20024479
Sufficient for 24 reactions when paired with AmpliSeq Library PLUS for Illumina. Purchase library prep, index adapters, and accessory products separately. View less
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set B for Illumina®
20019106
AmpliSeq™ CD Indexes Set C for Illumina®
20019107
AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately. View less
Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels). View less
AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing. View less
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Specifications

General

Assay time	5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Hands-on time	< 1.5 hr
Input quantity	10–1,000 ng
Content specifications	RNA evaluation of TCRβ chain rearrangements, including CDR1, CDR2, and CDR3 (with up to 400 bp read-length amplicons)
Instruments	MiSeq System , NextSeq 550 System , NextSeq 2000 System , NextSeq 1000 System , MiniSeq System
Mechanism of action	Multiplex PCR
Method	Amplicon sequencing , Targeted RNA sequencing
Multiplexing	96 dual index combinations
Nucleic acid type	RNA
Sample type details	Libraries can be generated from RNA extracted from whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS)-sorted cells.
Specialized sample types	Blood
Species category	Human
Technology	Sequencing
Variant class	Transcript variants

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

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