Description
Overview
NextSeq 500/550 v2.5 Kits deliver the power of high-throughput sequencing on the NextSeq 550 System. The v2.5 kits retain the same pre-filled, ready-to-use reagent cartridges as the v2 kits and offer improvements that provide increases in stability, robustness, and performance. The v2.5 kits offer:
An optimized flow cell that maintains starting fluorescent intensity, resulting in improved performance throughout the run
A more resilient flow cell able to withstand higher humidity, heat, and prolonged storage
Continued use of proven v2 reagent and buffer cartridges for exceptional accuracy for a broad range of methods
High- and mid-output sequencing options to support dynamic capacity needs and faster turnaround times
Radio-frequency identification (RFID)–encoded consumables to ensure reagent traceability and compatibility
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.
Configurations for multiple applications
The NextSeq 550 System allows researchers to perform a broad range of applications, from mRNA-Seq to exome sequencing and more. View recommended reagent kits for popular sequencing applications. All NextSeq 500/550 kits are paired-end capable.
NextSeq 500/550 Kits: High output Up to 400 million clusters
300 cycles: de novo Exome
de novo
Exome
Targeted panels
150 cycles: Whole transcriptome mRNA-Seq Exome
Whole transcriptome
mRNA-Seq
75 cycles: Gene expression profiling
Gene expression profiling
NextSeq 500/550 High Output Kit v2.5 (75 Cycles)
20024906
Provides kitted reagents for 75 cycles of sequencing, plus dual-indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (75 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge. View less
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NextSeq 500/550 High Output Kit v2.5 (150 Cycles)
20024907
Provides kitted reagents for 150 cycles of sequencing, plus dual- indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (150 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge. View less
NextSeq 500/550 High Output Kit v2.5 (300 Cycles)
20024908
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (300 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge. View less
NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles)
20024904
Provides kitted reagents for 150 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 130M reads). Includes: Mid Output Reagent Cartridge (150 cycles), Mid Output Flow Cell Cartridge, and Buffer Cartridge. View less
NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles)
20024905
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 130M reads). Includes: Mid Output Reagent Cartridge (300 cycles), Mid Output Flow Cell Cartridge, and Buffer Cartridge. View less
TG NextSeq™ 500/550 High Output Kit v2.5 (75 Cycles)
20024911
TG NextSeq™ 500/550 High Output Kit v2.5 (150 Cycles)
20024912
TG NextSeq™ 500/550 High Output Kit v2.5 (300 Cycles)
20024913
TG NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles)
20024909
TG NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles)
20024910
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How many samples can you load in one run using NextSeq 500/550 v2.5 Kits?
Users can sequence 1–12 exomes, 1–16 transcriptomes, 6–96 targeted panels, or 12–40 gene expression profiling samples per run on the NextSeq 550 System.
What is a NextSeq 550 flow cell?
The NextSeq 550 flow cell is a non-patterned flow cell with distinct nanowells of varied sizes, undefined shapes, and irregular spacing for use in cluster generation. Prepared libraries are loaded onto the flow cell, which is then loaded into the NextSeq 550 System for cluster generation and sequencing.
How many flow cells can be run on a NextSeq 550 System?
One flow cell can be run on the NextSeq 550 System.
Which control software is required for NextSeq v2.5 reagent kits?
An update to NextSeq Control Software (NCS 2.2.0) is required to run the NextSeq v2.5 sequencing reagent kits.
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