20025874, AmpliSeq™ for Illumina®, Sequencing Service

Name: AmpliSeq™ for Illumina®, Sequencing Service
Brand: Illumina
SKU: 20025874

AmpliSeq for Illumina custom sequencing project in Illumina Lab for 96–384 samples to support research projects (priced per sample). Includes pre-project consult, Illumina scientist support, experimental design, sample shipment coordination, library preparation and sample QC, sequencing, data analysis on BSSH and/or sequencing run files, technology transfer, fixed or custom panel, and consumables. Turnaround time is 6–8 weeks.

Catalog Number: 20025874

Pricing

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Category

NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

Accurate data across various sample types
Achieve unmatched data quality with AmpliSeq chemistry and Illumina next-generation sequencing (NGS) technology, even from low-quality starting materials such as formalin-fixed, paraffin-embedded (FFPE) tissues.

Include only the genes and targets of interest
Easily design custom content with DesignStudio, a free, user-friendly, online tool that creates panels optimized for the targets or specific genomic content of interest.
Flexible and tunable designs from 12 to over 12,000 amplicons, and multiple amplicon size options.

Fast, simple workflow
Increased efficiency with a 5.5-hour library preparation workflow with only 1.5 hours of hands-on time.

Scalable solution
Multiple options for sequencing platforms, data analysis, and support meet virtually any throughput requirements.

Example workflow
DesignStudio Software
AmpliSeq for Illumina Custom DNA Panel
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq System
BaseSpace Sequence Hub DNA Amplicon App
Local Run Manager DNA Amplicon Analysis Module
BaseSpace Variant Interpreter

Related applications and methods
Targeted Resequencing
With targeted sequencing or resequencing, a subset of genes or a genomic region of interest is isolated and sequenced, which can conserve lab resources.
AmpliSeq for Illumina Community Panels
These predesigned sequencing panels contain content selected and designed with input from leading disease researchers.
Oncology
Illumina NGS and microarray technologies for cancer research are helping drive the revolution in cancer genomics.

Figures
DNA Amplicon LRM Module
AmpliSeq™ Custom DNA Panel for Illumina®
20020495
Custom assay supporting 750 or 3000 samples with < 4999 amplicons. Purchase Library PLUS and index adapters separately. View less
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AmpliSeq™ Custom DNA Large Panel for Illumina®
20020497
Sufficient for 3000 samples with > 4999 amplicons. Purchase Library PLUS and index adapters separately. View less
AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set B for Illumina®
20019106
AmpliSeq™ CD Indexes Set C for Illumina®
20019107
AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately. View less
AmpliSeq™ for Illumina® Sample ID Panel
20019162
Includes eight single nucleotide polymorphism (SNP)-targeting primer pairs and one gender discriminating primer pair sufficient for 96 reactions when paired with AmpliSeq Library PLUS for Illumina. Enables quick and accurate sample identification. Purchase library prep, probe panels, and index adapters separately. View less
AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification. View less
AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing. View less
AmpliSeq™ for Illumina® DNA Library Preparation Kit Training
20023392
1 day on-site training for the complete AmpliSeq™ for Illumina DNA library preparation workflow. This training can be delivered with any of the available AmpliSeq™ for Illumina DNA panels. Includes quantification of DNA input as well as best practices, troubleshooting tips, and training on Illumina-supported analysis tools specific to the DNA application workflow. View less
AmpliSeq™ for Illumina®, Sequencing Service
20025874
AmpliSeq for Illumina custom sequencing project in Illumina Lab for 96–384 samples to support research projects (priced per sample). Includes pre-project consult, Illumina scientist support, experimental design, sample shipment coordination, library preparation and sample QC, sequencing, data analysis on BSSH and/or sequencing run files, technology transfer, fixed or custom panel, and consumables. Turnaround time is 6–8 weeks. View less
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Specifications

General

Assay time	As low as 5 hr (library prep only; doesn't include library quantification, normalization, or pooling time)
Hands-on time	1.5 hours
Input quantity	1–100 ng (10 ng recommended per pool)
Content specifications	Custom content of interest - up to 5 Mb
Description	A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.
Instruments	MiSeq System , iSeq 100 System , NextSeq 550 System , NextSeq 2000 System , NextSeq 1000 System , MiSeqDx in Research Mode , MiniSeq System , NextSeq 550Dx in Research Mode
Mechanism of action	Multiplex PCR
Method	Custom sequencing , Targeted DNA sequencing , Amplicon sequencing , Genotyping by sequencing
Multiplexing	Up to 96-plex
Nucleic acid type	DNA
Number of amplicons	12 to 12,288 amplicons
Number of reactions	750 or 3000 reactions
Specialized sample types	Blood, FFPE tissue
Species category	Any species, Tomato, Bovine, Mouse, Canine, Rice, Ovine, Hamster, Chicken, Porcine, Human, Maize, Soybean

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.