Description
Overview
NovaSeq 6000 Reagent Kits enable tunable output and proven performance for a broad range of applications on the NovaSeq 6000 system. These kits offer:
Ready-to-use reagent cartridges for streamlined operation
Various read lengths to easily adjust output and sample throughput
Multiple flow cell configurations to support an extensive output range
Increase lab efficiency
Improvements to the NovaSeq 6000 Reagent Kits include an enhanced reagent chemistry to streamline the workflow and accommodate more complex indexing strategies, an extended reagent shelf life of six months to provide project planning flexibility, and radio-frequency identification (RFID) to ensure reagent traceability and compatibility.
Flow cell configurations
SP and S1 provide the fastest run times and are ideal for smaller batch sizes or in situations where rapid turnaround time is required.
S2 offers a quick, powerful, and cost-effective option for high-throughput applications.
S4 delivers high throughput while enabling cost-effective sequencing across a range of applications and depth of coverage.
Additional benefits
NovaSeq 6000 Reagent Kits v1.5 also provide an improved Q30 quality score compared to the v1.0 reagents, support for unique molecular identifiers (UMIs), and support for whole-genome sequencing with Illumina DNA PCR-Free Library Prep.
Are NovaSeq 6000 flow cells available in single-read and paired-end versions?
NovaSeq 6000 Reagent Kits support both single-read and paired-end sequencing. During run setup, a run can be configured to generate single-read or paired-end data.
Can I use custom primers on the NovaSeq 6000 System?
Yes. For NovaSeq reagents v1.5, follow the NovaSeq Series Custom Primers Guide .
How many reads does a NovaSeq 6000 S4 flow cell provide?
The NovaSeq 6000 S4 flow cell provides 8–10B single reads and 16–20B paired-end reads.
How many reads does a NovaSeq 6000 SP flow cell provide?
The NovaSeq SP flow cell provides 650–800M single reads and 1.3–1.6B paired-end reads.
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