Illumina, 20031371, iSeq 100 i1 Reagent v2 (300-cycle)

Name: iSeq 100 i1 Reagent v2 (300-cycle)
Brand: Illumina
SKU: 20031371

Includes one i1 flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the iSeq 100 System.

Catalog Number: 20031371

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Category

General Purpose Incubators

TagsSequencing kits Clinical research products Sequencing reagents & flow cells

Description

Overview
The TruSight Hereditary Cancer Panel delivers expert-defined content for assessing germline mutations.
Extensive panel includes 113 genes associated with hereditary cancer predisposition
Easy protocol enables fast and flexible library prep and enrichment
Compatibility with all Illumina benchtop sequencing systems with sample throughput ranging from 2 to 256 samples per run
Hybrid–capture enrichment enables good coverage uniformity for accurate detection of SNVs, indels, and CNVs

Expert-defined content
The panel was developed with input from key opinion leaders and includes 10,341 probes across 113 genes and 125 SNPs.

Fast library prep and enrichment workflow
The panel is used with the Illumina DNA Prep with Enrichment library prep workflow, which takes advantage of enzyme-based DNA fragmentation, removing the instrument cost and need for mechanical fragmentation. Illumina DNA Prep with Enrichment has been qualified with multiple automation systems for a user-friendly workflow.

Scalable
Indexes available for up to 384 samples allow for flexible batch sizes and scalability across Illumina benchtop sequencing systems.

Applications
TruSight Hereditary Cancer Panel is a highly efficient targeted next-generation sequencing (NGS) panel that enables researchers to access an expert-defined content set for analyzing variation within genes previously linked with a predisposition towards cancer.

Example workflow
TruSight Hereditary Cancer – Enrichment Oligos
Illumina DNA Prep with Enrichment
Illumina DNA/RNA UD Indexes
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq 550 System
DRAGEN Enrichment
BaseSpace Enrichment

Project recommendations
Instrument: Recommended number of samples
iSeq 100 System: Samples per run: 2 (based on 300× mean coverage)
Samples per run: 2 (based on 300× mean coverage)
2 × 150 bp (max recommended)
MiniSeq System: Samples per run: mid-output: 5, high-output: 16 (based on 300× mean coverage)
Samples per run: mid-output: 5, high-output: 16 (based on 300× mean coverage)
MiSeq System: Samples per run: v2 Micro: 2, v2 Standard: 9, v3 Standard: 16 (based on 300× mean coverage)
Samples per run: v2 Micro: 2, v2 Standard: 9, v3 Standard: 16 (based on 300× mean coverage)
NextSeq 500 System: Samples per run: mid-output: 80, high-output: 256 (based on 300× mean coverage)
Samples per run: mid-output: 80, high-output: 256 (based on 300× mean coverage)
NextSeq 550 System: Samples per run: mid-output: 80, high-output: 256 (based on 300× mean coverage)

TruSight Hereditary Cancer Panel gene content
Includes 10,341 probes that target 113 genes related to cancer predisposition, recommended in key guidelines, and evaluated on population studies of cases vs. controls.

Genes included in key guidelines associated with risk reduction
The TruSight Hereditary Cancer Panel includes an extensive list of genes commonly associated with hereditary predisposition to breast, colon, ovarian, and gastric cancers and recommended in key guidelines.
TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions)
20029551
TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions) View less
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What cancer types are covered by the TruSight Hereditary Cancer Panel?
The TruSight Hereditary Cancer Panel includes an extensive list of genes commonly associated with hereditary predisposition to breast, colon, ovarian, and gastric cancers.

What Illumina sequencing systems can run the TruSight Hereditary Cancer Panel?
The TruSight Hereditary Cancer Panel can be run on any benchtop Illumina sequencing system (iSeq 100, MiSeq, MiniSeq, and NextSeq Systems).

How many SNPs are included in the TruSight Hereditary Cancer Panel?
TruSight Hereditary Cancer Panel includes 125 SNPs. Of these, 48 are ID SNPs and 77 are used for polygenic risk scoring (PRS).
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Specifications

General

Assay time	~48 hr from DNA to data
Hands-on time	~2 hr
Input quantity	50–1000 ng DNA
Automation capability	Liquid handling robot(s)
Cancer type	Pan-cancer, Solid tumor
Content specifications	Targets 113 cancer risk-associated genes
Description	The TruSight Hereditary Cancer Panel is a targeted sequencing panel designed to assess germline mutations across 113 genes and 125 single nucleotide polymorphisms (SNPs) for identification purposes and polygenic risk scoring.
Instruments	MiSeq System , iSeq 100 System , NextSeq 550 System , MiniSeq System , NextSeq 550Dx in Research Mode , NextSeq 500 System , MiSeq i100 Plus System , MiSeq i100 System
Method	Targeted DNA sequencing , Target enrichment
Nucleic acid type	DNA
Sample type details	Genomic DNA
Specialized sample types	Blood, Saliva
Species category	Human
Technology	Sequencing
Variant class	Germline variants, Insertions-deletions (indels), Copy number variants (CNVs)

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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