20031675, AmpliSeq™ for Illumina® TCR beta-SR Panel

Name: AmpliSeq™ for Illumina® TCR beta-SR Panel
Brand: Illumina
SKU: 20031675

Sufficient for 24 DNA or RNA samples when paired with AmpliSeq Library PLUS for Illumina. Includes AmpliSeq dNTP Mix for Illumina. Purchase library prep, index adapters, and accessory products separately.

Catalog Number: 20031675

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Category

NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

Overview
AmpliSeq for Illumina TCR beta-SR (Short Read) Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples. It is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. You can use a single pool of multiplex PCR primers, library reagents, and sample barcodes to generate libraries from DNA/RNA extracted from FFPE, whole blood, fresh-frozen tissue, or fluorescence activated cell sorting (FACS)-sorted cells.

Key features include:
High-value content - Obtain comprehensive coverage of TCR beta chain sequences
Simple, streamlined workflow - Prepare sequencing-ready libraries in a single day from FFPE, whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS) cells
Low input requirement - Generate high-quality sequencing libraries from as little as 10 ng of input RNA
The AmpliSeq for Illumina TCR beta-SR Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. It enables characterization of the hypervariable complementary determining region 3 (CDR3) of the TCR beta chain. This information can help immunology researchers understand immune function, autoimmune diseases, and immune-mediated adverse effects.
Learn More About AmpliSeq for Illumina

Example workflow
AmpliSeq for Illumina TCR beta-SR Panel
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
AmpliSeq for Illumina cDNA Synthesis
MiniSeq System
MiSeq System
NextSeq 550 System

Project recommendations
Instrument: Recommended number of samples
MiniSeq System: Samples per run will depend on sample type.
Samples per run will depend on sample type.
2 × 151 bp
MiSeq System: Samples per run will depend on sample type.
NextSeq 550 System: Samples per run will depend on sample type.

Related applications and methods
Amplicon sequencing
Ultra-deep sequencing of PCR amplicons enables analysis of specific genomic regions of interest. Learn more about amplicon sequencing and find comprehensive solutions.
Cancer genomics research
Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.
Immunogenomics research
Gain insight into the functional impact of immune-related genetic variation and potential immunological disease mechanisms with NGS and array solutions.

Figures
AmpliSeq™ for Illumina® TCR beta-SR Panel
20031675
Sufficient for 24 DNA or RNA samples when paired with AmpliSeq Library PLUS for Illumina. Includes AmpliSeq dNTP Mix for Illumina. Purchase library prep, index adapters, and accessory products separately. View less
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List Price:
Discounts:
AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set B for Illumina®
20019106
AmpliSeq™ CD Indexes Set C for Illumina®
20019107
AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately. View less
Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels). View less
AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing. View less
AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification. View less
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Specifications

General

Assay time	5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Hands-on time	<1.5 hr
Input quantity	1 ng
Automation capability	Liquid handling robot(s)
Cancer type	Solid tumor
Content specifications	Sequences TCR beta chain rearrangements, with up to 80 bp read-length amplicons for characterizing CDR3.
Instruments	MiSeq System , NextSeq 550 System , NextSeq 2000 System , NextSeq 1000 System , MiniSeq System
Mechanism of action	Multiplex PCR
Method	Targeted DNA sequencing , Amplicon sequencing , Targeted RNA sequencing
Nucleic acid type	DNA, RNA
Number of amplicons	Varies
Specialized sample types	Blood, FFPE tissue
Species category	Human
Target insert size	80 bp
Technology	Sequencing
Variant class	Transcript variants

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

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