Description
Overview
The AmpliSeq for Illumina Custom RNA Fusion Panel enables detection of fusion genes and gene expression profiling in 12 to 1200 gene targets in a single assay. Targeted panels can be designed from a menu of over 1000 well-annotated fusion genes and > 20,000 human RefSeq genes using DesignStudio—a web-based assay design software tool.
Key features
Fast gene fusion detection - Prepare libraries in 6 hours with < 1.5 hours hands-on time. Results are available in < 2 days.
Low-quality, low-quantity sample input - Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.
RNA-to-analysis solution - Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.
An AmpliSeq for Illumina Custom RNA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
Example workflow
AmpliSeq for Illumina Custom RNA Panel
DesignStudio Software
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq 550 System
BaseSpace Sequence Hub RNA Amplicon App
Local Run Manager RNA and RNA Modules
BaseSpace Variant Interpreter
Related applications and methods
Amplicon Sequencing
Ultra-deep sequencing of PCR amplicons enables analysis of specific genomic regions of interest. Learn more about amplicon sequencing and find comprehensive solutions.
Cancer RNA Sequencing
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
High call rate for gene fusions
Custom Fusion Panel A (+12 Gene Expression Targets): Custom Fusion Panel B (+28 Gene Expression Targets)
RNA Source: Seraseq Fusion RNA Mix v3
EML4-ALK: •
KIF5B-RET: •
NCOA4-RET: -
CD74-ROS1: •
SLC34A2-ROS1: •
TPM3-NTRK1: •
FGFR3-BAIAP2L1: •
PAX8-PPARG1: -
FGFR3-TACC3: -
ETV6-NTRK3: •
LMNA-NTRK1: -
SLC45A3-BRAF: •
TMPRSS2-ERG: •
EGFR-SEPT14: •
Reference fusions detected in panel (expected/observed): 10/10
AmpliSeq™ Custom RNA Fusion Panel for Illumina®
20032798
Targeted custom RNA fusion panel. 12 to 1,200 amplicons. Sufficient for 1,125 reactions. Library PLUS and index adapters sold separately. View less
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Discounts:
AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately. View less
AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set B for Illumina®
20019106
AmpliSeq™ CD Indexes Set C for Illumina®
20019107
AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately. View less
AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately. View less
Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels). View less
AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing. View less
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