Description
Overview
Reagents provided in the Illumina Ribo-Zero Plus rRNA Depletion Kit remove abundant RNA, such as ribosomal RNA and human globin transcripts, using enzymatic depletion. The remaining RNA is then converted into sequencing-ready libraries for Illumina sequencing systems using any stranded RNA prep.
Illumina Ribo-Zero Plus rRNA Depletion
Sample: rRNAs Targeted
Human cytoplasmic rRNAs: 28S, 18S, 5.8S, 5S
Human mitochondrial rRNAs: 12S, 16S
Human beta globin transcripts: HBA1, HBA2, HBB, HBG1, HBG2
Mouse & Rat rRNA: 16S, 28S
Gram(-) Bacterial rRNAs: E. coli: 5S, 16S, 23S
Gram(+) Bacterial rRNAs: Bacillus subtilis: 5S, 16S, 23S
Applications
The Illumina Ribo-Zero Plus rRNA Depletion Kit is a streamlined RNA-to-analysis solution for sequencing high-value, informative portions of the transcriptome.
Related applications and methods
RNA sequencing
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA.
Total RNA sequencing
Analyze both coding RNA and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.
Ribosomal RNA depletion
Compare key features of ribosomal RNA (rRNA) and globin mRNA depletion kits. View sample type compatibility and the rRNA types removed by each kit.
Figures
Multispecies ribodepletion
Ribo-Zero Plus rRNA Depletion Kit effectively depletes rRNA levels in a variety of species. Comparison data between Illumina Stranded Total RNA Prep with Ribo-Zero Plus and TruSeq Stranded Total RNA paired with Ribo-Zero Gold or Ribo-Zero Bacteria (B. subtilis data not shown).
Effective globin mRNA depletion
Globin mRNA depletion from human peripheral blood leukocytes with Illumina Stranded Total RNA Prep with Ribo-Zero Plus using different RNA inputs.
Illumina® Ribo-Zero Plus rRNA Depletion Kit (16 Samples)
20040526
Includes Ribo-Zero Plus depletion module for depletion of ribosomal RNA from human, mouse, rat, and bacteria and globin transcripts for 96 samples. Purchase purification beads, library prep reagents and index adapters separately. For complete kit, including library prep reagents, order the Illumina Stranded Total RNA with Ribo-Zero Plus kit. View less
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Illumina Ribo-Zero plus rRNA Depletion Kit (96 Samples)
20037135
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Does the Illumina Ribo-Zero Plus rRNA Depletion Kit deplete globin mRNA from non-human total RNA?
The Illumina Ribo-Zero Plus rRNA Depletion Kit depletes globin mRNAs from human blood samples. The mouse and rat globin mRNAs with homologous sequence with human's globin mRNAs are also removed. Use of this kit with other organisms has not been tested and is not supported.
Is there a custom probe design option for Illumina Ribo-Zero Plus rRNA Depletion Kit?
There is an option to design custom supplemental spike-in probes for species not covered in the standard module. Please consult your local Sequencing Specialist to learn more about this option.
What molecules are removed with the Illumina Ribo-Zero Plus rRNA Depletion Kit?
The kit removes a mix target of cytoplasmic rRNA, mitochondrial rRNA, and globin mRNA.
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