Illumina, 20042146, Infinium Methyl Custom-24 Kit (48 Spl)

Name: Infinium Methyl Custom-24 Kit (48 Spl)
Brand: Illumina
SKU: 20042146

Infinium HTS iSelect Methyl Custom Beadchip, 48 samples

Catalog Number: 20042146

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Category

ChIP Reagents & Kits

Pack Size24 Kit

TagsMicroarray Kits

Description

Infinium HTS iSelect Methyl Custom BeadChip
Fully customizable methylation microarray for large, targeted epigenetic applications.
Infinium HTS iSelect Methyl Custom BeadChip Data sheet PDF < 1 MB
Data sheet PDF < 1 MB
Sample throughput
Number of samples
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Overview
The Infinium Custom Methylation Kit contains the Infinium HTS iSelect Methyl Custom BeadChip plus reagents to enable researchers and commercial users to develop groundbreaking assays for critical epigenetic research applications.

High-throughput workflow
The Infinium HTS iSelect Methyl Custom BeadChip is offered in a 24-sample format with updated Infinium automation protocols and an automated workflow for bisulfite conversion. These features improve workflow efficiency, decrease sample processing costs, and allow users to scale their operations up to hundreds of thousands of samples per year.

Customizable content
Customize arrays with up to 100K additional markers to create unique research tools. Select content from existing commercial arrays or epigenetic databases.

Proven Infinium chemistry
The Infinium BeadChip design enables a high degree of methylation probe replication, resulting in high reproducibility, comparable to 100× coverage in methylation sequencing experiments. 1

Key features of the Infinium Custom Methylation Kit
Customizable content supports up to 100K markers
Accurate quantitative measurement of DNA methylation at the single-CpG-level
Rapid turnaround time with optional automated bisulfite conversion

Applications
The Infinium Custom Methylation Kit allows users to create custom high-throughput assays to measure DNA methylation in targeted regions of the genome for academic epigenetic applications, including common disease research, cancer research, epigenetic aging, and nutriepigenomics.

Example workflow
Methylation arrays
Methylation arrays enable high-throughput, quantitative interrogation of methylation sites across the genome at single-nucleotide resolution.
iScan System
The iScan microarray scanner supports high-throughput BeadChip processing, scanning hundreds to thousands of samples quickly and accurately.
GenomeStudio software
GenomeStudio software provides an integrated platform for data analysis of microarray-based genotyping assays, with a user-friendly graphical interface.

Related applications and methods
Epigenetics
Illumina offers solutions for epigenetic analysis. Use our tools to study epigenetic modifications and their impact on gene regulation.
DNA methylation analysis
Genome-wide methylation profiling studies with microarray and NGS technologies can provide novel insights into the functional impact of methylation.
High-throughput genotyping
Large-scale genotyping with microarrays can identify variants associated with disease risk in large cohorts or populations.

Infinium I and II assays allow for broader coverage of CpG sites
Illumina methylation BeadChips employ both Infinium I and Infinium II assays, enhancing the overall breadth of coverage. (A) Infinium I probe designs use 2 bead types per CpG locus, 1 each for the methylated and unmethylated states. (B) Infinium II probe designs use 1 bead type for both states.

Design and manufacturing process
Researchers work with Illumina technical design and customer service teams to refine the BeadChip marker content and coordinate the ordering and production process.
Zhou L, Ng HK, Drautz-Moses DI, et al. Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing. Sci Rep. 2019;9(1):10383. Published 2019 Jul 17. doi:10.1038/s41598-019- 46875-5

Resources
Infinium Methyl Custom-24 Kit (48 Spl)
20042146
Infinium HTS iSelect Methyl Custom Beadchip, 48 samples View less
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Inf Methyl EX iSelect-48 Kit (48 Spl)
20139002
This kit contains 1 custom BeadChip and reagents to process 48 samples throught the Infinium EX Methylation workflow. View less
Inf Methyl EX iSelect-48 Kit (96 Spl).
20139003
This kit contains 2 custom BeadChips and reagents to process 96 samples throught the Infinium EX Methylation workflow. View less
Inf Methyl EX iSelect-48 Kit (1152 Spl).
20139004
This kit contains 24 custom BeadChip and reagents to process 1152 samples throught the Infinium EX Methylation workflow. View less
Inf Methyl EX iSelect-24 Kit (48 Spl).
20139005
This kit contains 2 custom BeadChip and reagents to process 48 samples throught the Infinium EX Methylation workflow. View less
Inf Methyl EX iSelect-24 Kit (96 Spl).
20139006
This kit contains 4 custom BeadChip and reagents to process 96 samples throught the Infinium EX Methylation workflow. View less
Infinium® Assay: An Introduction – Customer Site
20015273
Three-day, hands-on instruction at customer site to familiarize users with the essential steps in the Infinium protocol. Course provides hands-on training in sample and BeadChip preparation, sample scanning using the iScan System, and primary data evaluation and analysis using array analysis software. View less
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How do I analyze the Infinium HTS iSelect Methyl Custom BeadChip data?
For methylation QC, GenomeStudio 2011, Methylation Module, or DRAGEN Array Methyl QC can be used for human species.
For downstream methylation analysis or analysis of non-human species, SeSAMe ( https://www.bioconductor.org/packages/release/bioc/html/sesame.html ) or other third party bioconductor tools are recommended at this time.

Which bisulfite conversion kit should I use?
The Zymo Research EZ-96 Methylation-Lightning MagPrep Kit in manual and automated formats is supported for use upstream to Infinium Methylation assays. Review results in this technical note for more details.
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References
Zhou L, Ng HK, Drautz-Moses DI, et al. Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing. Sci Rep. 2019;9(1):10383. Published 2019 Jul 17. doi:10.1038/s41598-019- 46875-5

Specifications

General

Sample throughput	5760 samples per week max (with 2 iScan systems, 1 AutoLoader 2x, and 2 Tecan robots over an 8-hour day)
Number of samples	24 samples per array
Number of markers	500-100,000 user defined CpGs add-on capacity
Assay type	Infinium HTS
Automation capability	Automated array loader
Description	Using the DesignStudio Assay Design Tool, this product allows labs to create high-throughput, custom, 24- or 48-sample BeadChips to efficiently and accurately assess methylation for up to 100,000 CpG sites in human and select nonhuman samples.
Input quantity	250 ng DNA
Instruments	iScan System
Method	Methylation array
Nucleic acid type	DNA
Specialized sample types	Blood, Cell-free DNA, Saliva
Species category	Mouse, Human
Species details	Select non-human species available on DesignStudio only
Technology	Microarray
Variant class	Differentially methylated cytosines

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

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