Description
NovaSeq 6000 Xp Workflow
NovaSeq 6000 Xp Workflow delivers the flexibility of independent lane loading and high library multiplexing per flow cell, maximizing sample throughput on a single run.
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Overview
The NovaSeq 6000 Xp Workflow supports a variety of applications, such as whole-genome sequencing (WGS), ultradeep exome sequencing, and single-cell sequencing. Improving upon the standard workflow, the NovaSeq 6000 Xp Workflow adds a level of flexibility and control to experimental planning and flow cell configuration on the NovaSeq 6000 System.
Key features
Separation of projects or methods by lane
Ability to run incompatible library types on the same flow cell
Multiplexing within each lane to maximize total number of samples per flow cell
Lower concentration of denatured libraries
Maximize flexibility and efficiency
The NovaSeq 6000 Xp Workflow offers the flexibility to load each flow cell lane independently and multiplex samples within each lane. These features maximize the total number of samples and reduce the required DNA input per flow cell without compromising data quality or yield.
Related applications and methods
Large whole-genome sequencing
Large whole-genome sequencing informs disease research and population genomics studies and reveals disease-associated alleles.
Exome sequencing
Whole-exome sequencing is used to investigate protein-coding regions of the genome to uncover genetic influences on disease and population health.
Shotgun metagenomic sequencing
Comprehensively sample all genes in all organisms in complex samples to evaluate bacterial diversity and detect unculturable microorganisms using NGS.
NovaSeq 6000 Xp Workflow Course
NovaSeq XP 4-Lane Kit v1.5
20043131
Includes three tubes of ExAmp reagents and a single manifold for loading a four-lane NovaSeq flow cell. Used along with the NovaSeq Xp Flow Cell Dock in an optional workflow that allows accessibility to individual lanes of the NovaSeq flow cell. View less
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NovaSeq XP 2-Lane Kit v1.5
20043130
Includes three tubes of ExAmp reagents and a single manifold for loading a two-lane NovaSeq flow cell. Used along with the NovaSeq Xp Flow Cell Dock in an optional workflow that allows accessibility to individual lanes of the NovaSeq flow cell. View less
NovaSeq XP 2-Lane Kit
20021664
The NovaSeq Xp 2-Lane Kit is a consumable used along with the NovaSeq Xp Flow Cell Dock in an optional workflow that allows accessibility to individual lanes of the NovaSeq flow cell. The kit consists of ExAmp reagents (3 tubes) and a single manifold needed to load a 2-lane NovaSeq flow cell. View less
NovaSeq Xp 4-Lane Kit
20021665
The NovaSeq Xp 4-Lane Kit is a consumable used along with the NovaSeq Xp Flow Cell Dock in an optional workflow that allows accessibility to individual lanes of the NovaSeq flow cell. The kit consists of ExAmp reagents (3 tubes) and a single manifold needed to load a 4-lane NovaSeq flow cell. View less
NovaSeq Xp Flow Cell Dock
20021663
Includes one reusable flow cell holder for use with all NovaSeq flow cells, required for the optional NovaSeq Xp workflow. View less
NovaSeq™ Xp 2-Lane Manifold Pack
20021666
Includes spare pack with 16 additional 2-lane manifolds for use with the NovaSeq Xp workflow. View less
NovaSeq™ Xp 4-Lane Manifold Pack
20021667
Includes spare pack with 16 additional 4-lane manifolds for use with the NovaSeq Xp workflow. View less
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Can I expect similar data quality for the standard and NovaSeq 6000 Xp Workflow?
Yes. Data quality is expected to be equivalent. Please refer to this application note for details.
How does NovaSeq 6000 Xp Workflow loading work?
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