Description
Overview
The TruSight RNA Pan-Cancer Panel offers in-depth and cost-effective assessment of cancer-related RNA transcripts in limited and degraded samples on a benchtop sequencing system.
Industry-validated content for a comprehensive view of 1385 genes implicated in cancer pathways
Comprehensive view of cancer pathways enabling interrogation of gene expression levels, variants, and gene fusions
Low-input protocol for most sample types, including formalin-fixed, paraffin-embedded (FFPE) samples
Efficient analysis of difficult samples
Libraries can be prepared from as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity.
Intuitive cloud-based data analysis
Analysis can be performed using the BaseSpace RNA-Seq Alignment App , which provides gene expression profiles, identifies gene fusions, and reports single nucleotide variants and small indels, offering a comprehensive, intuitive solution to cancer researchers.
Sample data sets
Register or sign in to BaseSpace Sequence Hub to access MiSeq System data and MiniSeq System data ( view run or view project ).
Applications
The TruSight RNA Pan-Cancer Panel provides comprehensive assessment of cancer-related RNA transcripts and fusion detection in FFPE tissue and other oncology samples for research.
Example workflow
Targeted RNA Sequencing
MiniSeq System
MiSeq System
BaseSpace RNA-Seq Alignment App
Project recommendations
Instrument: Recommended number of samples
MiniSeq System: Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample)
Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample)
2 × 75 bp (max recommended)
MiSeq System: 8 samples per run with v3 reagents (based on 3 million reads per sample)
8 samples per run with v3 reagents (based on 3 million reads per sample)
Related applications and methods
Pan-cancer analysis
Pan-cancer analysis with NGS comprehensively assesses gene variants and abnormalities associated with many common cancers, regardless of tumor origin.
Cancer RNA sequencing
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
Low-quality/FFPE RNA-Seq solutions
Find FFPE RNA sequencing solutions that yield high-quality results from degraded FFPE tissues and other low-quality samples.
Figures
TruSight Pan-Cancer capture chemistry
The TruSight RNA PanCancer Panel provides a simple, streamlined method for isolating targeted regions of interest from total RNA.
Identification of novel gene fusion partners
(A) The size distribution of total RNA from 3 different FFPE samples derived from melanoma are shown on a Bioanalyzer. (B) Table shows the detection of the gene fusion partner in FFPE samples when only one gene (highlighted) of the fusion pair is targeted.
TruSight RNA Pan-Cancer Panel Set A
RS-303-1002
Includes library preparation reagents and oligos for 48 samples with 12 indexes; each index is sufficient for 4 individual samples. May be used with TruSight RNA Pan-Cancer Panel Set B to allow for pooling up to 24 samples. View less
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TruSight RNA Pan-Cancer Panel Set B
RS-303-1003
Includes library preparation reagents and oligos for 48 samples with 12 indexes; each index is sufficient for 4 individual samples. May be used with TruSight RNA Pan-Cancer Panel Set A to allow for pooling up to 24 samples. View less
TruSight RNA Pan-Cancer Set A MiniSeq Kit
20005611
Includes library preparation reagents and oligos for 48 samples with 12 indexes, plus 6 MiniSeq High Output Kits (150 Cycles). View less
Trusight RNA Pan-Cancer Oligo Panel
20046104
Enrichment oligos only. Library prep and index adapter reagents need to be ordered separately View less
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How does TruSight RNA Pan-Cancer work?
The TruSight RNA Pan-Cancer workflow involves six major steps:
Generating stranded RNA-Seq libraries
Hybridizing biotinylated probes to targeted regions
Capturing targets using streptavidin beads
Eluting captured fragments from beads
Generating clusters and sequencing
Analyzing data and calling gene fusions with BaseSpace RNA Alignment App
Learn more .
What probe pool does the TruSight RNA Pan-Cancer Panel use?
The probes are designed to target 1385 cancer genes and detect fusions by spanning all exons of every gene.
How do I run my data through the RNA-Seq Alignment App?
Run the RNA-Seq workflow (FASTQ only) on the MiSeq System and stream the data to BaseSpace Sequence Hub. The BaseSpace RNA-Seq Alignment App analyzes data from the TruSight RNA Pan-Cancer Panel, providing a simple results summary that includes a fusion table, variant table, and gene expression table. You may also use your own pipeline for analysis.
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