Description
Overview
The Illumina Ribo-Zero Plus Microbiome rRNA Depletion Kit is a streamlined RNA-to-analysis solution for metatranscriptome sequencing of complex microbial samples, including stool.
Key features
Perform deeper analysis and gain insights into microbial activity by reducing unwanted reads and increasing coverage of expressed bacterial RNA.
Eliminate unwanted rRNA from bacteria common in the adult and infant human gut, as well as host RNA from human, mouse, using proven Ribo-Zero Plus enzymatic ribodepletion technology.
Analyze complex metatranscriptomes with complimentary access to the BaseSpace Microbiome Metatranscriptomics app.
Example workflow
Ribo-Zero Plus Microbiome rRNA Depletion Kit
Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome
NextSeq 550 System
NextSeq 1000/2000 Systems
NovaSeq 6000 System
BaseSpace Microbiome Metatranscriptomics app
Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: Mid output: 2 High output: 8 (based on 25-50 million reads per sample)
Mid output: 2 High output: 8 (based on 25-50 million reads per sample)
2 x 75 bp
NovaSeq 6000 System: Whole Transcriptome Analysis: SP: 16 S1: 32 S2: 82 S4: 200 samples per run (based on 25-50 million reads per sample)
Whole Transcriptome Analysis: SP: 16 S1: 32 S2: 82 S4: 200 samples per run (based on 25-50 million reads per sample)
2 × 100 bp
Related applications and methods
Human microbiome analysis
Learn how NGS facilitates microbial community profiling studies, and explore methods and solutions for analyzing the human microbiome.
Microbial genomics
Next-generation sequencing (NGS) is changing microbial genomics. Use NGS to discover novel microbes, monitor outbreaks, analyze food sources, and more.
Host-pathogen interaction analysis
Study host and pathogen gene expression and analyze how the microbiome influences responses to pathogens and vaccines with next-generation sequencing.
Figures
The Ribo-Zero Plus Microbiome Depletion kit effectively depletes rRNA (5S, 16S, and 23S) levels for human and bacterial species in a single-tube reaction. Results are compared to Ribo-Zero Plus rRNA Depletion Kit and a commercially available ribodepletion kit from Company X. Two stool mixtures were tested. Sample 1 is a mixture containing both adult and infant human stool samples, while Sample 2 is a commercially available stool sample from Zymo (Cat no. 6331).
Resources
See how Drs. Tonya Ward, Emily Hollister, and Le François of Diversigen and DNA Genotek developed workflows for metatranscriptomic studies and read their views for this research area’s potentially impactful future.
See Spotlight
In this webinar, you'll hear from three leading experts in the field as they share their insights and key considerations on sample collection and processing, rRNA depletion, and bioinformatic analysis.
Watch webinar
Illumina® Ribo-Zero Plus rRNA Microbiome depletion kit (96 Samples)
20072062
Includes Ribo-Zero Plus Microbiome depletion module for depletion of ribosomal RNA from diverse bacteria commonly found in microbiomes and targets included in the Ribo-Zero Plus kit for 96 samples. For complete kit including library prep reagents, order the Illumina Stranded Total RNA with Ribo-Zero Plus Microbiome kit. View less
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Illumina® Stranded Total RNA Prep with Ligation, Ribo-Zero Plus Microbiome (96 Samples)
20072063
Includes reagents and Ribo-Zero Plus Microbiome depletion module for preparing 96 libraries. Compatible only with IDT for Illumina RNA UD indexes, Ligation. Indexes sold separately. View less
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