20076914, Twist Bioscience® for Illumina Exome 2.5 Panel

Name: Twist Bioscience® for Illumina Exome 2.5 Panel
Brand: Illumina
SKU: 20076914

Twist Bioscience for Illumina Exome 2.5 Panel provides 68 µl of material. When paired with Illumina FFPE DNA Prep with Exome 2.5 Enrichment, this volume is sufficient for 20 enrichment reactions at 4- plex for manual workflow. Available by quote only, panel must be sold with library prep reagents. Request pricing for more details.

Catalog Number: 20076914

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Category

NGS Library Preparation Kits

Pack Sizebox of 96

TagsSequencing kits Library preparation kits

Description

Overview
Illumina FFPE DNA Prep with Exome 2.5 Enrichment is a sensitive and comprehensive whole-exome sequencing solution that offers a tumor-normal workflow for detecting low-frequency variants from FFPE (formalin-fixed, paraffin-embedded) samples.
The workflow includes unique molecular identifiers (UMIs) for error correction and reduction of false positives, enabling accurate detection of low-frequency mutations.

Key features
Comprehensive exome coverage for tumor-normal workflows
Streamlined sample-to-insights workflow with automation-friendly fill volumes
Accurate detection of low-frequency mutations
Illumina FFPE DNA Prep with Exome 2.5 Enrichment detects low-frequency variants, including single nucleotide variants (SNVs), indels, and copy number variants (CNVs). The kit identifies small variants with allele frequencies as low as 5% from only 40 ng FFPE DNA with over 90% variant recall. The Twist Biosciences for Illumina Exome 2.5 Panel content includes biomarkers for tumor mutational burden (TMB), microsatellite instability (MSI), and homologous recombination deficiency (HRD).
This kit is part of an integrated workflow that starts with FFPE DNA from tumors and matched normal samples. After Illumina sequencing, obtain highly accurate variant calling using the DRAGEN Somatic App.
Illumina FFPE DNA Prep with Exome 2.5 Enrichment offers a fast, hybrid-capture workflow for preparing sequencing-ready libraries in ~10 hours, with only 4 hours of hands-on time. Researchers can go from extracted FFPE DNA to sequencing in a single day. The recommended read length is 2 × 151 bp.
This kit maximizes sequencing results, enabling thorough coverage of exomes using a focused, comprehensive, and up-to-date panel.
Illumina custom v2 probes make it easy to add mitochondrial genome coverage or regions of interest using spike-in panels with up to 10,000 probes.
Illumina FFPE DNA Prep with Exome 2.5 Enrichment features the same library preparation and enrichment chemistry as Illumina Cell-Free DNA with Enrichment to enable exceptional performance with FFPE samples.

Applications
Illumina FFPE DNA Prep with Exome 2.5 Enrichment is a streamlined, comprehensive sample-to-insights whole-exome sequencing solution that supports low-input samples, including FFPE DNA.

Example workflow
Illumina FFPE DNA Prep with Exome 2.5 Enrichment
Illumina Custom Enrichment Panel v2
NextSeq 2000 System
NovaSeq 6000 System
NovaSeq X Series
DRAGEN Somatic
DRAGEN Enrichment

What panels do I need?
Twist Bioscience for Illumina Exome 2.5 Panel is a comprehensive and up-to-date exome panel that allows uniform coverage for whole-exome sequencing.
Twist Bioscience for Illumina Mitochondrial Panel is an optional fixed content spike-in panel that allows enrichment and sequencing of mitochondrial DNA variants.
Illumina Custom Enrichment Panel v2 can be used as spike-in panels, using the DesignStudio Assay Design Tool or with the Illumina Concierge design team.
Illumina® Cell-Free and FFPE DNA Prep, Ligation (16 Samples)
20104105
Includes ligation-based library prep for preparation of 16 libraries with Illumina Qualified Automation Methods, or 24 libraries manually. Enrichment reagents and enrichment panel not included. UMI Index Adapters are purchased separately and required for library preparation. Choose based on prep method. Please note regarding data analysis: Cell-Free DNA: Analyze data on DRAGEN for ILMN Cell-Free DNA Prep with Enrichment App on Illumina Connected Analytics or BaseSpace Sequence Hub at no additional charges. Basic subscription to Cloud platform required. Data storage not included. FFPE DNA: Analysis on DRAGEN Enrichment and Somatic not included. View less
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Illumina® Cell-Free and FFPE DNA Prep, Ligation (96 Samples)
20104106
Includes ligation-based library prep for preparation of 96 libraries with Illumina Qualified Automation Methods, or 120 libraries manually. Enrichment reagents and enrichment panel not included. UMI Index Adapters are purchased separately and required for library preparation. Choose based on prep method. Please note regarding data analysis: Cell-Free DNA: Analyze data on DRAGEN for ILMN Cell-Free DNA Prep with Enrichment App on Illumina Connected Analytics or BaseSpace Sequence Hub at no additional charges. Basic subscription to Cloud platform required. Data storage not included. FFPE DNA: Analysis on DRAGEN Enrichment and Somatic not included. View less
Illumina® Cell-Free and FFPE DNA Prep, Enrichment (16 Reactions)
20104107
Includes fast enrichment reagents for enrichment of Illumina Cell-Free and FFPE DNA Prep libraries for a total of 16 reactions with Illumina Qualified Automation Methods, or 24 reactions manually. Library preparation reagents and enrichment panel not included. UMI Index Adapters are purchased separately and required for library preparation. Choose based on prep method. Cell-Free DNA: 1-4 samples per reaction during enrichment. Analyze data on DRAGEN for ILMN Cell-Free DNA Prep with Enrichment on Illumina Connected Analytics or BaseSpace Sequence Hub at no additional charge. Basic subscription to Cloud platform required. Data storage not included. FFPE DNA: 4 samples per Whole Exome enrichment. Analysis on DRAGEN Enrichment and Somatic not included. View less
Illumina® Cell-Free and FFPE DNA Prep with Enrichment, Ligation (192 Samples, 4-plex), Cloud Analysis
20104103
Includes ligation based library prep and fast enrichment reagents for preparation of 192 libraries using Illumina Qualified Automation Methods, or 240 libraries manually, using 4-plex enrichment (pooling of 4 libraries in a single enrichment reaction). UMI Index Adapters are purchased separately and required for library preparation. Choose based on prep method. Enrichment panel not included. Please note regarding data analysis: Cell-Free DNA: Analyze data on DRAGEN for ILMN Cell-Free DNA Prep with Enrichment on Illumina Connected Analytics or BaseSpace Sequence Hub at no additional charges. Basic subscription to Cloud platform required. Data storage not included. FFPE DNA: Analysis on DRAGEN Enrichment and Somatic not included. View less
Illumina® Cell-Free and FFPE DNA Prep with Enrichment, Ligation (192 Samples, 4-plex), On-premises
20104104
Includes ligation based library prep and fast enrichment reagents for preparation of 192 libraries using Illumina Qualified Automation Methods, or 240 libraries manually, using 4-plex enrichment (pooling of 4 libraries in a single enrichment reaction). UMI Index Adapters are purchased separately and required for library preparation. Choose based on prep method. Enrichment panel not included.10,000 Gb, 1 yr DRAGEN Server License included. Illumina DRAGEN v4 Server required for Cell- free DNA analysis. View less
Twist Bioscience® for Illumina Exome 2.5 Panel
20076914
Twist Bioscience for Illumina Exome 2.5 Panel provides 68 µl of material. When paired with Illumina FFPE DNA Prep with Exome 2.5 Enrichment, this volume is sufficient for 20 enrichment reactions at 4- plex for manual workflow. Available by quote only, panel must be sold with library prep reagents. Request pricing for more details. View less
Twist Bioscience for Illumina Mitochondrial Panel
20093180
Twist Bioscience for Illumina Mitochondrial Panel can be used for combined enrichment of both human exome plus mitochondrial DNA from whole exome libraries. Provides 32 ul of material, sufficient for 96 samples when paired with Exome 2.5 Panel in the Illumina DNA Prep with Exome 2.5 Enrichment kits (8 hybridization reactions at 12-plex with up to 4 ul oligo panel per reaction), or a minimum of 64 samples when paired with Exome 2.5 Panel in the lllumina FFPE DNA Prep with Exome 2.5 Enrichment kit (16 hybridization reactions at 4-plex with 2 ul oligo panel per hybridization reaction). View less
Illumina Custom Enrichment Panel V2 (32 µl, 120 bp)
20073953
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 32 µl of material (sufficient for 8 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately. View less
Illumina Custom Enrichment Panel V2 (384 µl, 120 bp)
20073952
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 384 µl of material (sufficient for 96 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately. View less
Illumina Custom Enrichment Panel V2 (1536 µl, 120 bp)
20111339
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 1536 µl of material (sufficient for 384 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately. View less
IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)
20034701
UMI DNA Index Anchors (Plate = 20027219, Box = 20032119) + Nextera Compatible Unique Dual Index A (Sales Kit = 20027213, Plate = 20025019, Box = 20026121) View less
IDT® for Illumina® UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)
20034702
UMI DNA Index Anchors (Plate = 20027219, Box = 20032119) + Nextera Compatible Unique Dual Index B (Sales Kit = 20027214, Plate = 20025080, Box = 20026930) View less
IDT for Illumina UMI DNA/RNA Index Anchors Set A for Automation
20066404
Includes one box of 96 IDT for Illumina - UMI DNA/RNA UD Indexes Set A for Automation sufficient for labeling 96 samples and one box of 96 IDT for Illumina - UMI DNA Index Anchors for Automation. View less
IDT for Illumina UMI DNA/RNA Index Anchors Set B for Automation
20063213
Includes one box of 96 IDT for Illumina - UMI DNA/RNA UD Indexes Set B for Automation sufficient for labeling 96 samples and one box of 96 IDT for Illumina - UMI DNA Index Anchors for Automation. View less
Illumina UMI DNA/RNA UD v3 indexes Set A, Ligation (96 indexes, 96 Samples)
20126235
Includes one box of 96 Illumina DNA/RNA UD v3 Indexes Set A sufficient for labeling 96 samples and one box of 96 IDT for Illumina UMI DNA Index Anchors. View less
Illumina UMI DNA/RNA UD v3 indexes Set B, Ligation (96 indexes, 96 Samples)
20126237
Includes one box of 96 Illumina DNA/RNA UD v3 Indexes Set B sufficient for labeling 96 samples and one box of 96 IDT for Illumina UMI DNA Index Anchors. View less
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Specifications

General

Assay time	~10 hr
Hands-on time	~4 hr
Input quantity	40 ng FFPE
Cancer type	Solid tumor
Description	A fast and easy-to-use library prep with an enrichment tumor–normal workflow for accurate and comprehensive whole-exome sequencing and reliable detection of low-frequency mutations from FFPE samples.
Input quantity	40 ng FFPE DNA
Instruments	NextSeq 2000 System , NovaSeq X System , NovaSeq 6000 System , NovaSeq X Plus System
Method	Exome sequencing
Nucleic acid type	DNA
Specialized sample types	Low-input samples, FFPE tissue
Species category	Human
Technology	Sequencing
Variant class	Single nucleotide polymorphisms (SNPs), Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

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