Overview
TruSight Oncology 500 High-Throughput (HT) is a highly scalable pan-cancer, next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from FFPE tissue.
Targets 523 genes to assess all DNA and RNA variant types (SNV, CNV, indels, fusions), plus MSI and TMB
Delivers accurate results with hybrid–capture chemistry and sophisticated bioinformatics
Reduces hands-on time and increases scalability with optional automation kits and methods*
Accommodates 8-36 samples with the NextSeq 1000 and NextSeq 2000 Systems , 16-192 samples with the NovaSeq 6000 System , and 32-960 samples with the NovaSeq X Series per run
Meaningful results
Evaluate > 500 pan-cancer biomarkers aligned with guidelines and clinical trials to increase chances of finding a positive biomarker with a single assay.
Streamlined workflow
Get results in 4–5 days using manual or automated workflows integrated with ready-to-use DRAGEN secondary analysis pipelines via on-premises server or in the cloud with Illumina Connected Analytics. Access an integrated interpretation report through Illumina Connected Insights † or Velsera Clinical Genomics Workspace (CGW ).
* Automation available on the Beckman Coulter iSeries or the Hamilton Microlab STAR . Contact Illumina Sales for more information.
† Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
Enable CGP with a comprehensive portfolio
Identify relevant biomarkers for clinical research with the TruSight Oncology 500 product line.
TruSight Oncology 500 High-Throughput
Batch up to 960 samples at a time while using the same panel content and tissue input type as TruSight Oncology 500.
TruSight Oncology 500
Assess relevant DNA and RNA cancer biomarkers from FFPE tumor tissue, now including HRD through an accessory kit to evaluate the Genomic Instability Score (GIS).‡
TruSight Oncology 500 ctDNA
Analyze circulating tumor DNA (ctDNA) in blood plasma via liquid biopsy with similar DNA panel content as TruSight Oncology 500.
‡ HRD is only available with the addition of the TruSight Oncology 500 HRD kit to TruSight Oncology 500. Not available in Japan.
§ The NextSeq 1000 System, NextSeq 2000 System, NovaSeq 6000Dx instrument (in Research Mode), and NovaSeq X Series require a separate, stand-alone DRAGEN server for secondary analysis.
Applications
TruSight Oncology 500 High-Throughput (HT) enables comprehensive genomic profiling of FFPE tumor samples to assess a wide range of biomarkers at increased scalability, from 8–480 samples per flow cell.
Example workflow
TruSight Oncology 500 High-Throughput assay
NextSeq 1000 & 2000 Systems
NovaSeq 6000 System
NovaSeq6000 Dx (in research mode)
NovaSeq X Series
Variant calling:
DRAGEN secondary analysis: on-premises via DRAGEN server
DRAGEN secondary analysis: cloud-based via Illumina Connected Analytics (ICA)
TruSight Oncology 500 Local App: Docker-based software
Insights and reporting:
Illumina Connected Insights**
** Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
Project recommendations
Instrument: Recommended number of samples
NextSeq 1000 System, NextSeq 2000 System: 8 samples per run (P2 flow cell), 800M paired-end reads, 3,500× coverage 24 samples per run (P3 flow cell), 2.4B paired-end reads, 3,500× coverage 36 samples per run (P4 flow cell), 3.6B paired-end reads, 3,500× coverage
8 samples per run (P2 flow cell), 800M paired-end reads, 3,500× coverage
24 samples per run (P3 flow cell), 2.4B paired-end reads, 3,500× coverage
36 samples per run (P4 flow cell), 3.6B paired-end reads, 3,500× coverage
2 x 101 bp
NovaSeq 6000 System: 16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage 32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage 72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage 192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage
16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage
32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage
72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage
192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage
2 × 101 bp
NovaSeq 6000Dx in Research Mode: 16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage 32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage 72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage 192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage
NovaSeq X System: 32 samples per run (1.5B flow cell), 3.2B paired-end reads, 3,500× coverage 192 samples per run (10B flow cell), 20B paired-end reads, 3,500× coverage 480 samples per run (25B flow cell), 52B paired-end reads, 3,500× coverage *Throughput shown is for the single flow cell NovaSeq X Instrument. NovaSeq X Plus offers a dual flow cell system with twice the capacity listed.
32 samples per run (1.5B flow cell), 3.2B paired-end reads, 3,500× coverage
192 samples per run (10B flow cell), 20B paired-end reads, 3,500× coverage
480 samples per run (25B flow cell), 52B paired-end reads, 3,500× coverage
*Throughput shown is for the single flow cell NovaSeq X Instrument. NovaSeq X Plus offers a dual flow cell system with twice the capacity listed.
Related applications and methods
Library prep automation
Reduce hands-on time and minimize errors with high- and low-throughput walk-away automation protocols available through Illumina automation partners.
Immuno-oncology research
Next-generation sequencing enables immuno-oncology researchers to gain insights into immunotherapy response factors and tumor immune evasion mechanisms.
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
Robust detection of fusions and splice variants
The information in this table shows examples of concordance between TruSight Oncology 500 and TruSight Oncology 500 High-Throughput and is not a comprehensive list of the fusions and splice variants detected. These data were generated with a Local App pipeline (not DRAGEN software).
CNV (Gene Amplification) Concordance
High concordance between NovaSeq X Series and NovaSeq 6000 for TSO 500 HT
Automation with TruSight Oncology 500 HT
Hear how customers at Intermountain Precision Genomics enabled CGP and scaled up their workflow with automation.
TruSight Oncology 500 DNA High-Throughput (48 Samples)
20040765
Includes reagents for preparing and enriching up to 48 DNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately. View less
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TruSight Oncology 500 DNA High-Throughput (144 Samples)
20040767
Includes reagents for preparing and enriching up to 144 DNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately. View less
TruSight Oncology 500 DNA/RNA High-Throughput (24 Samples)
20040764
Includes reagents for preparing and enriching up to 24 DNA and RNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately. View less
TSO 500 DNA/RNA HT (24 Spl) plus Illumina Connected Insights Software
20119460
Includes library prep and enrichment reagents plus data interpretation reports (through ICI). View less
TruSight Oncology 500 DNA/RNA High-Throughput (72 Samples)
20040766
Includes reagents for preparing and enriching up to 72 DNA and RNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately. View less
TruSight Oncology 500 DNA/RNA High-Throughput Kit (24 Samples), plus Velsera
20040768
Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately. View less
TruSight Oncology 500 DNA High-Throughput Kit (48 Samples), plus Velsera
20040769
Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately. View less
TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples), plus Velsera
20040770
Includes reagents for preparing and enriching up to 72 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately. View less
TruSight Oncology 500 DNA High-Throughput Kit (144 Samples), plus Velsera
20040771
Includes reagents for preparing and enriching up to 144 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately. View less
TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples)
20049283
Includes reagents for preparing and enriching up to 64 DNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation. View less
TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples)
20049282
Includes reagents for preparing and enriching up to 32 DNA and RNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation. View less
TSO500 DNA/RNA HT Auto (32 spls)+ plus Illumina Connected Insights Software
20119461
TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples), Plus Velsera
20049277
Includes reagents for preparing and enriching up to 64 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation. View less
TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples), Plus Velsera
20049276
Includes reagents for preparing and enriching up to 32 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation. View less
TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples)
20049285
Includes reagents for preparing and enriching up to 144 DNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation. View less
TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples), Plus Velsera
20049279
Includes reagents for preparing and enriching up to 144 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation. View less
TruSight Oncology 500 High-Throughput DNA/RNA for Automation (72 Samples)
20049284
Includes reagents for preparing and enriching up to 72 DNA and RNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation. View less
TSO500 DNA/RNA HT Auto (72 spls) + Velsera
20049278
Includes reagents for preparing and enriching up to 72 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation. View less
IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)
20034701
UMI DNA Index Anchors (Plate = 20027219, Box = 20032119) + Nextera Compatible Unique Dual Index A (Sales Kit = 20027213, Plate = 20025019, Box = 20026121) View less
IDT® for Illumina® UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)
20034702
UMI DNA Index Anchors (Plate = 20027219, Box = 20032119) + Nextera Compatible Unique Dual Index B (Sales Kit = 20027214, Plate = 20025080, Box = 20026930) View less
IDT for Illumina UMI DNA/RNA Index Anchors Set A for Automation
20066404
Includes one box of 96 IDT for Illumina - UMI DNA/RNA UD Indexes Set A for Automation sufficient for labeling 96 samples and one box of 96 IDT for Illumina - UMI DNA Index Anchors for Automation. View less
IDT for Illumina UMI DNA/RNA Index Anchors Set B for Automation
20063213
Includes one box of 96 IDT for Illumina - UMI DNA/RNA UD Indexes Set B for Automation sufficient for labeling 96 samples and one box of 96 IDT for Illumina - UMI DNA Index Anchors for Automation. View less
NovaSeq™ X Series 1.5B Reagent Kit (200 Cyc)
20104704
This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems. View less
NovaSeq X Series 10B Reagent Kit (200 Cycle)
20085595
This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems. View less
NovaSeq™ X Series 25B Reagent Kit (300 Cyc)
20104706
This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems. View less
NovaSeq 6000 S4 Reagent Kit v1.5 (200 cycles)
20028313
Includes one S4 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System. View less
NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles)
20028315
Includes one S2 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System. View less
NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles)
20028318
Includes one S1 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System. View less
NovaSeq 6000 SP Reagent Kit v1.5 (200 cycles)
20040719
Includes one SP flow cell on,e buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System. View less
NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100986
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 400M single reads). View less
NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100989
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads). View less
NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100993
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads). View less
Trusight Oncology 500 HT Training (DNA) - Customer Site
20042220
Three-day, hands-on instruction at customer site to familiarize users with TruSight Oncology 500 HT (DNA only). Course provides hands-on training in library preparation, enrichment, sequencing, and data analysis for a maximum of four trainees. NOTE: The short description should be updated as well. We no longer use "TST". It should be consistent with others and say "TSO 500." View less
Trusight Oncology 500 HT Training (DNA and RNA) - Customer Site
20042219
3.5-day, hands-on instruction at customer site to familiarize users with TruSight Oncology 500 HT (DNA and RNA). Course provides hands-on training in library preparation, enrichment, sequencing, and data analysis for a maximum of four trainees. NOTE: The short description should be updated as well. We no longer use "TST". It should be consistent with others and say "TSO 500." View less
Connected Insights Training - Remote
20092376
Illumina Connected Insights Training - Remote includes five (5) hours of product training delivered virtually. View less
Informatics Professional Services - Remote
20071787
Professional services for Illumina informatics products and solutions including software implementations, pipelines and workflow development, integration, networking, migration, data ingestion projects and other informatics consultative, project-based services, performed remotely, and defined by a statement of work. View less
Illumina DRAGEN Server v3
20040619
Includes Avance Exchange support for the first year. Requires purchase of annual DRAGEN license. View less
Illumina DRAGEN Server v4
20051343
Includes Advance Exchange support for the first year. Requires purchase of annual DRAGEN license. View less
ICA Basic Annual Subscription
20044874
Illumina Connected Analytics (ICA) Basic Annual Subscription. This product includes 1 year of access to ICA Basic, including sequencing instrument connectivity, data management capabilities, and access to pre-packaged analysis tools. View less
ICA Professional Annual Subscription
20044876
Illumina Connected Analytics (ICA) Professional Annual Subscription. This product includes 1 year of access to ICA, including sequencing instrument connectivity, data management capabilities, access to pre- packaged tools, and the ability to create customized workflows composed of tools, pipelines, data warehouses, and notebooks. View less
ICA Enterprise Annual Subscription
20038994
Illumina Connected Analytics (ICA) Enterprise Annual Subscription. This product includes 1 year of access to ICA Enterprise, including sequencing instrument connectivity, data management capabilities, custom and pre-packaged analysis tools, and the Base module for data warehousing and mining. ICA Enterprise also includes optional HIPAA BAA (US-only), single sign-on (SSO), and a service level agreement (SLA). View less
ICA Enterprise Srvc & Compliance Add-on
20066830
Illumina Connected Analytics (ICA) Compliance enables single sign-on (SSO), multi-factor authentication (MFA), HIPAA BAA (US-only), and a Service Level Agreement (SLA) for an ICA Basic Annual Subscription. View less
Illumina Analytics - 1 iCredit
20042038
iCredits are used for data storage and analysis on either BaseSpace Sequence Hub or Illumina Connected Analytics. View less
Illumina Analytics Starter Pack - 1,000 iCredits
20042039
Illumina Analytics - 5,000 iCredits
20042040
Illumina Analytics - 50,000 iCredits
20042041
Illumina Analytics - 100,000 iCredits
20042042
Insights - Annual Subscription
20090137
Annual subscription to Illumina Connected Insights platform. View less
Illumina Connected Insights – Oncology Genome Equivalent Sample - VCF
20090138
Illumina Connected Insights pre-paid oncology samples on per genome equivalent basis starting from VCF: one genome is equivalent to 2 exomes, 3 large and 6 small panel samples. Any unused samples will automatically roll over provided that the annual subscription to Illumina Connected Insights (20090137) is renewed on an annual basis. Access to a set of oncology knowledge bases is included. View less
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How does TruSight Oncology 500 HT differ from the TruSight Oncology 500 assay?
The primary difference between the assays is scale. TruSight Oncology 500 HT runs on a variety of sequencers: NextSeq 1000 and 2000, NovaSeq 6000, NovaSeq 6000Dx (in Research Mode), and the NovaSeq X Series. Sample batching can range from 8-960 samples at a time, providing scalability to higher sample throughput. TruSight Oncology 500 runs on the NextSeq 550 System or NextSeq 550Dx Instrument (in Research Mode) and can batch up to 8 samples at a time. See the comparison table on this page for other differences.
Can I automate the TSO 500 HT workflow?
Yes, you can automate the TSO 500 HT workflow with Illumina Qualified automation methods . Illumina Qualified methods are available on the Beckman Coulter Life Sciences i7 and the Hamilton STAR (MOA configuration) liquid handling robots. Illumina Qualified methods are co-developed by Illumina and the automation partner. Automation specific library prep kits are offered by Illumina to accommodate the reagent volume required by automation platforms.
Can TSO 500 HRD be added to the TSO 500 HT library prep?
TruSight Oncology 500 HRD is not currently validated for use with TruSight Oncology 500 High-Throughput. Please reach out to your local Illumina sales or support representatives for information on alternative solutions. TruSight Oncology 500 HRD is not available in Japan.
What are the benefits of using DRAGEN for variant calling (secondary analysis)?
The Illumina DRAGEN secondary analysis platform delivers greater variant calling accuracy at twice the speed of non-DRAGEN options. 1 Increased accuracy provides better performance for sample QC, complex variants, small variants, fusions, and splice variants, as well as exon-level CNV calling for BRCA1/BRCA2. Illumina will continue to bring innovative features to DRAGEN TruSight Oncology 500 pipelines.
Can I analyze TruSight Oncology 500 HT data with my own software?
Yes, you can perform analysis with your own software. However, Illumina will not be able to directly provide technical support in this case.
Frequently purchased
Other products to consider
References
Data calculations on file. Illumina, Inc. 2023.
