20100469, Illumina DNA/RNA UD Index Set A.

Name: Illumina DNA/RNA UD Index Set A.
Brand: Illumina
SKU: 20100469

Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set A. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.

Catalog Number: 20100469

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NGS Library Preparation Kits

TagsSequencing kits Library preparation kits

Description

Illumina Respiratory Virus Enrichment Kit
A streamlined workflow offering highly sensitive detection and characterization of common respiratory viruses, including SARS-CoV-2 strains.
Assay time
Hands-on time
Input quantity
The Respiratory Virus Oligo Panel v2 (RVOP v2, Catalog no. 20044311) is now sold in comprehensive kits (Sets A-D) that include the Respiratory Virus Oligo Panel V2, library prep and enrichment reagents, and index adapters. The standalone RVOP v2 product or individual components can be purchased through MyIllumina .
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Overview
The Illumina Respiratory Virus Enrichment Kit, formerly known as the Respiratory Virus Oligo Panel (RVOP), provides an effective, streamlined method for detecting common respiratory viruses, such as SARS-CoV-2 and influenza A/B.
Understand variant analysis for viral evolution or viral surveillance 1
Determine viral transmission patterns and predict resurgence
Identify and characterize co-infections and their role in complex diseases

Target enrichment for respiratory virus detection
Target enrichment through the hybrid–capture method allows for highly sensitive detection without requiring the high read depth needed for shotgun metagenomic sequencing. Additionally, this method allows for dramatically larger probe panels for comprehensive profiling compared to amplicon sequencing while producing whole-genome sequencing data for the targeted viruses.

Streamlined solution for viral applications
This comprehensive workflow integrates library preparation, target enrichment, sequencing, and data analysis, giving researchers and public health scientists the ability to monitor multiple viruses and variants with one workflow

Applications
Illumina Respiratory Virus Enrichment Kit, Illumina sequencing, and DRAGEN secondary analysis offer a streamlined workflow that enables the detection and characterization of over 40 common human respiratory viruses.

Example workflow
llumina Respiratory Virus Enrichment Kit
NextSeq 550 System
NextSeq 1000/2000 System
MiSeq i100
DRAGEN RNA Pathogen Detection
DRAGEN Microbial Enrichment Plus

Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: High output: 384 samples, 1M clusters and a minimum of 1M single-end reads per sample

Related applications and methods
Target enrichment
Target enrichment captures genomic regions of interest through hybridization. Learn more about target enrichment and how it can be used to assess multiple samples at once.
Coronavirus sequencing
NGS can identify novel coronavirus variants, track COVID-19 transmission, and more. Compare NGS methods for various coronavirus sequencing goals.
Infectious disease research
As a hypothesis-free method, NGS can distinguish between infectious disease strains that differ by as little as one SNP, and replace multiple tests.

Enrichment workflow for respiratory virus detection
The streamlined NGS workflow for respiratory virus detection integrates sample preparation, library preparation, target enrichment, sequencing, and data analysis.

Highly sensitive SARS-CoV-2 detection
In a titration experiment of human saliva RNA spiked with a defined amount of virus copies, the Illumina Respiratory Virus Enrichment Kit resulted in detection of viral reads from as low as two copies of SARS-CoV-2 viral RNA.

Viruses detected by Illumina Respiratory Virus Enrichment Kit
The Respiratory Virus Oligo Panel includes 7800 probes to detect common respiratory viruses, recent flu strains, SARS-CoV-2, and human probes to act as positive controls. All probes are 80-mer oligos, spaced very close together, providing full genome coverage of all viruses in the panel.
Human coronavirus 229E: Human parainfluenza virus 1
Human coronavirus NL63: Human parainfluenza virus 2
Human coronavirus OC43: Human parainfluenza virus 3
Human coronavirus HKU1: Human parainfluenza virus 4a
SARS-CoV-2: Human metapneumovirus (CAN97-83)
Human adenovirus B1: Respiratory syncytial virus (type A)
Human adenovirus C2: Human Respiratory syncytial virus 9320 (type B)
Human adenovirus E4: Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Human bocavirus 1 (Primate bocaparvovirus 1 isolate st2): Influenza A virus (A/Korea/426/1968(H2N2))
Human bocavirus 2c PK isolate PK-5510: Influenza A virus (A/New York/392/2004(H3N2))
Human bocavirus 3: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Illumina DNA/RNA UD Index Set A.
20100469
Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set A. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus. View less
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Illumina Respiratory Virus Enrichment Kit Set B (96 indexes, 96 samples)
20100470
Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set B. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus. View less
Illumina Respiratory Virus Enrichment Kit Set C (96 indexes, 96 samples)
20100471
Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set C. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus. View less
Illumina Respiratory Virus Enrichment Kit Set D (96 indexes, 96 samples)
20100472
Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set D. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus. View less
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Which data analysis solutions are recommended for the Illumina Respiratory Virus Enrichment Kit?
The Illumina Respiratory Virus Enrichment Kit includes free usage of the DRAGEN RNA Pathogen Detection app and the DRAGEN Microbial Enrichment Plus , both of which analyze pathogen data and generate consensus sequences.
Frequently purchased
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Specifications

General

Sample type details
Assay time	< 9 hr library prep time
Hands-on time	< 2 hr library prep time
Input quantity	10–100 ng
Automation capability	Liquid handling robot(s)
Automation details	Explore available automation methods
Content specifications	Targets and characterizes ~40 common respiratory viruses, including SARS-CoV-2, influenza A and B viruses, adenovirus, rhinovirus, respiratory syncytial virus, and other common respiratory viruses.
Description	A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses. The agnostic design allows for widespread identification of pathogenic viruses across multiple sample types of interest
Instruments	MiSeq System , NextSeq 550 System , NextSeq 2000 System , MiniSeq System , MiSeq i100 System , MiSeq i100 Plus System
Mechanism of action	On-bead tagmentation followed by a single hybridization step
Method	Targeted DNA sequencing , Targeted RNA sequencing , Target enrichment
Multiplexing	Up to 384 samples in a single run with unique dual indexes
Nucleic acid type	DNA, RNA

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