Description
Overview
Illumina Microbial Amplicon Prep—Influenza A/B is an amplicon-based library preparation kit built on the same chemistry as the COVIDSeq assay. Library preparation is fast and easy, generating libraries compatible with nearly all Illumina sequencing systems. This kit enables whole-genome sequencing of influenza A/B viruses for public health surveillance and influenza virus research. Analysis can be performed using the DRAGEN Targeted Microbial Amplicon on Basespace Sequence Hub with pre-loaded targets. Files can also be easily uploaded for custom analyses.
Expand your public health surveillance and influenza virus research capabilities
Get sequencing results with exceptional data quality and accuracy at any scale with fast turnaround times.
Key features
Multiplexed, automation-friendly RT-PCR and tagmentation-based workflow
Simplified data analysis and reporting with the DRAGEN Targeted Microbial App on BaseSpace Sequence Hub
Excellent performance with RNA from a range of sample types, including cultures and nasal swabs
This kit provides exceptional coverage across influenza A/B virus genomes, addressing sequencing challenges related to high genomic variability. In addition, achieve rapid, unbiased, and accurate influenza virus sequencing and characterization to support high-impact genomic surveillance and research efforts.
Example workflow
Illumina Microbial Amplicon Prep—Influenza A/B
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq 550 System
NextSeq 1000 & 2000 Systems
DRAGEN Microbial Amplicon
Related applications and methods
Public health surveillance
Genomics can advance public health and wellness by detecting and characterizing new, emerging, and circulating pathogens.
Genomic surveillance
NGS supports effective genomic surveillance strategies through identification of novel strains of coronavirus and other emerging infectious disease agents.
Microbial genomics
Next-generation sequencing (NGS) is changing microbial genomics. Use NGS to discover novel microbes, monitor outbreaks, analyze food sources, and more.
Fast, flexible library prep with IMAP
In this video, Illumina Scientist Jeff Koble provides an overview of the Illumina Microbial Amplicon Prep assay, detailing tips and tricks to help ensure successful preparation of libraries.
Simple and efficient IMAP analysis with DRAGEN Targeted Microbial App
Illumina Bioinformatics Scientist Soo Bin Kwon demonstrates how DRAGEN Target Microbial enables comprehensive analysis of microbial targets sequenced with IMAP.
Illumina® Microbial Amplicon Prep—Influenza A/B (48 samples)
20106305
Complete amplicon-prep based library prep kit for whole genome sequencing of Influenza A and B virus strains. Amplicon sequencing technology is based on well established COVIDSeq but optimized for Influenza A/B viral sequencing. Each kit provides enough reagents for 48 samples and includes library prep reagents, an influenza A/B targeting primer pool, and an index plate with 48 unique dual indexes. View less
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