Description
Overview
The Viral Surveillance Panel v2 Kit allows researchers to perform whole-genome sequencing (WGS) that can characterize a wide range of viruses, including SARS-CoV-2, influenza, hantaviruses, arboviruses, and hepatitis, with target enrichment.
Coverage of more than 200 viruses identified as high-risk to public health
Concurrent enrichment for RNA and DNA viruses
Compatible with a range of samples, including wastewater, environmental, and post-clinical
Target enrichment for virus detection
Target enrichment through a hybrid–capture method allows for WGS of multiple viruses, without requiring the high read depth needed for shotgun metagenomic sequencing, providing insight to viral evolution and broad viral surveillance. 1 Additionally, as compared to other targeted resequencing methods, such as amplicon sequencing, hybrid–capture provides greater ability to capture, sequence and identify mutations, making it ideal for outbreak surveillance of rapidly evolving viruses.
Streamlined solution for viral applications
This comprehensive workflow integrates library preparation, target enrichment, sequencing, and data analysis, allowing researchers and public health scientists the ability to monitor multiple high-risk viruses with one panel/workflow.
Applications
The Viral Surveillance Panel v2 Kit provides an optimized workflow that supports viral outbreak detection and monitoring of over 200 RNA and DNA viral pathogens.
Example workflow
Viral Surveillance Panel v2 Kit (Sets A-D)
Illumina RNA Prep with Enrichment
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq 550 System
NextSeq 1000 and 2000 Systems
DRAGEN Microbial Enrichment Plus
Related applications and methods
Genomic surveillance
NGS supports effective genomic surveillance strategies through identification of novel strains of coronavirus and other emerging infectious disease agents.
Coronavirus sequencing
NGS can identify novel coronavirus variants, track COVID-19 transmission, and more. Compare NGS methods for various coronavirus sequencing goals.
Wastewater surveillance
Learn about wastewater surveillance with NGS for community-level monitoring of infectious diseases, mutation tracking, and variant trend tracking.
Read counts and viral genome coverage gains
Compared to shotgun metagenomic sequencing, where all RNA/DNA is sequenced, targeted hybrid–capture reduces unnecessary sequencing of host and nontargeted microbes.
Uniform viral genome coverage
Target enrichment probes in the Viral Surveillance Panel v2 Kit provide uniform coverage of whole virus genomes. Virus controls were prepared by mixing virus controls at known copy numbers with 10 ng human RNA/DNA mix. Libraries were prepared and sequenced following the Viral Surveillance Panel v2 Kit workflow.
Wastewater surveillance
Viruses detected in wastewater using Viral Surveillance Panel v2 Kit or shotgun sequencing. a Samples with low viral concentrations are easily detectable when using the Viral Surveillance Panel v2 Kit.
See the data sheet or Virus List Spreadsheet for the full list of viruses detected.
Download the Viral Surveillance Panel v2 Virus List Spreadsheet
a. Samples were collected by researchers at Colorado State University and purified total nucleic acids were shipped to Illumina for testing. Libraries were prepared and sequenced using 100 ng total nucleic acids
The panel detects over 200 viruses and subtypes, including the 66 viruses identified as important risks to public health by the World Health Organization (WHO). 2
Included on the Viral Surveillance Panel
Adeno-associated virus 2: Human adenoviruses (species A - G)
Aichi virus 1: Human bocavirus
Aigai virus: Human coronaviruses (4 species)
Bombali virus: Human cytomegalovirus
Bourbon virus: Human immunodeficiency viruses (1 and 2)
Cache Valley virus: Human metapneumovirus
California encephalitis virus: Human papillomaviruses (28 types)
Chapare virus: Human parainfluenza viruses (types 1 - 4)
Chikungunya virus: Human parechovirus
Colorado tick fever virus: Human parvovirus B19
Coxsackieviruses (A and B): Influenza A virus (20 subtypes)
Crimean-Congo hemorrhagic fever virus: Influenza B virus (both lineages)
Dengue viruses (4 types): Influenza C virus
Ebola viruses (6 species): Jamestown Canyon virus
Echovirus: Japanese encephalitis virus
Enteroviruses (A - D): Junin virus
Epstein-Barr virus: Kyasanur Forest disease virus
Equine encephalitis viruses (3 species): La Crosse virus
Guanarito virus: Lassa virus
Hantaviruses (22 species): Lloviu virus
Heartland virus: Lujo virus
Hendra virus: Lymphocytic choriomeningitis virus
Hepatitis viruses (A - E): Lyssavirus
Herpes simplex viruses (1 and 2): Machupo virus
Zika virus
Illumina Viral Surveillance Panel v2 Kit, Set A (96 samples)
20108081
Viral Surveillance Panel V2 Kit (VSP V2) is a hybrid capture enrichment kit that provides whole genome sequencing of over 200 viruses and subtypes of public health concern. View less
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Illumina Viral Surveillance Panel v2 Kit, Set B (96 samples)
20108082
Illumina Viral Surveillance Panel v2 Kit, Set C (96 samples)
20108083
Illumina Viral Surveillance Panel v2 Kit, Set D (96 samples)
20108084
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What is the difference between Viral Surveillance Panel v1 and Viral Surveillance Panel v2?
Viral Surveillance Panel v1 provides results for 66 viruses of public health concern. Viral Surveillance Panel v2 expands this target list to over 200 viruses and subtypes. Viral Surveillance Panel v2 includes Illumina Purification Beads, which are not included in Viral Surveillance Panel v1.
What is the read depth recommendation for Viral Surveillance Panel v2-enriched libraries?
For good quality samples, the read depth is a minimum of 2M paired-end reads per sample with a read length of 150 bp. For more complex samples, such as wastewater, a minimum of 8M paired-end reads is recommended per sample.
Are both RNA and DNA viruses detected using the Viral Surveillance Panel v2 Kit?
Yes, dsRNA, ssRNA, dsDNA, and ssDNA viruses are detected.
What viruses are included in VSPv2?
Download the full list of viruses and subtypes in VSPv2 here .
