Description
Overview
The Infinium Methylation Screening Array is an affordable and highly scalable epigenomics tool for population health research.
Highly informative content for common disease in human populations
~270K unique methylation sites selected from mining over a decade of research from methylation arrays and sequencing studies and databases
Comprehensive coverage of DNA methylation sites implicated in genetic diseases, environmental exposures, aging, and development
Coverage of emerging functional genomics markers, including cell-type specific methylation, intermediate methylation, correlated regions of systemic interindividual variation (CoRSIVs), and more
Exceptional sample processing efficiency
Process up to 600,000 samples/year on a single iScan System with automation
Lower sample processing costs with 48-sample BeadChip and automation
Reliable methylation data and simple informatics
Precise, read-depth independent DNA methylation measurements results in > 98% beta value reproducibility between replicate samples
Straightforward data analysis with free, easy-to-use third-party packages
Applications
The Methylation Screening Array enables affordable, large-scale epigenetic analysis for population health studies.
Example workflow
Ex Methylation Assay
Infinium Automation Packages
iScan System
AutoLoader 2.x
Related applications and methods
DNA methylation analysis
Genome-wide methylation profiling studies with microarray and NGS technologies can provide novel insights into the functional impact of methylation.
Epigenetics
Illumina offers solutions for epigenetic analysis. Use our tools to study epigenetic modifications and their impact on gene regulation.
Methylation arrays
Methylation arrays enable high-throughput, quantitative interrogation of methylation sites across the genome at single-nucleotide resolution.
Figures
Highly reproducible methylation results—(A) Methylation results are highly correlated between Infinium Methylation Screening Array and Infinium MethylationEPICv2.0 BeadChips. (B) Methylation results demonstrate excellent reproducibility between replicate samples analyzed on the Infinium Methylation Screening Array.
Markers on the Infinium Methylation Screening Array are highly enriched in known trait-associated CpGs across diverse trait types—Markers on the Methylation Screening Array (MSA) are selected based on evidence for trait association, shown in comparison with trait enrichment on Infinium MethylationEPICv2.0 (EPICV2).
Infinium Methylation Screening Array-48 Targeted methylation screening for population health research
Recommended applications: Common disease research (noncancer) Environmental epidemiology Population genomics Consumer genomics
Content focus: Known common disease trait associations Known environmental exposure associations Cell-type specific methylation Intermediate methylation Multiomic capabilities to measure high MAF SNPs
Total methylation sites: 270K
Number of samples per BeadChip: 48
DNA input requirement: 50 ng
Assay chemistry: Infinium EX Methylation
Instrument support: iScan
iScan System maximum sample throughput a: 16,128 samples/week
Liquid Handling Automation: Infinium Automated Pipetting System with ILASS (required)
a Approximate values, scan times, and maximum throughput will vary depending on laboratory and system configurations. Sample throughput listed here is achieved with integration of AutoLoader 2.x automated array loading.
Infinium Methylation Screening Array: Infinium MethylationEPIC v2.0
Total unique sites: 269,094
CpG: 262,470
From Infinium methylation arrays: 161,569
From sequencing studies and databases: 101,901
CpH: 2776
From Infinium methylation arrays: 308
From sequencing studies and databases: 2468
SNP rsID: 3848
From Infinium methylation arrays: 64
From sequencing studies and databases: 3784
Trait category: No. of probes targeting trait association
Development/aging: 102,533
Environmental exposures: 44,043
Inflammation/autoimmune diseases: 41,894
Ancestry: 31,843
Sex: 23,806
Infectious diseases: 14,844
Metabolic diseases: 13,739
Rare genetic disorders: 13,429
Neurological/neurodevelopmental diseases: 8874
Body features (body morphology): 8109
Psychiatric disorders: 7280
Cardiovascular disease: 7007
Reproductive biology/health: 6999
Neurodegenerative diseases: 4733
Lung/respiratory diseases: 1748
Renal disease: 982
Infinium Methylation Screening Array-48 Kit (48 samples)
20112611
Optimized methylation array with 270K markers for high-throughput common disease research & screening. Kit contains 1 48-sample BeadChip and all required reagents for processing 48 samples after bisulfite conversion. Requires iScan System and Infini View less
Sign in to add to cart or see pricing.
List Price:
Discounts:
Infinium Methylation Screening Array-48 Kit (96 samples)
20112612
Optimized methylation array with 270K markers for high-throughput common disease research & screening. Kit contains 2 48-sample BeadChips and all required reagents for processing 96 samples after bisulfite conversion. Requires iScan System and Infin View less
Infinium Methylation Screening Array-48 Kit (1,152 samples)
20112613
Optimized methylation array with 270K markers for high-throughput common disease research & screening. Kit contains 24 48-sample BeadChips and all required reagents for processing 1,152 samples after bisulfite conversion. Requires iScan System and I View less
Inf Methyl Screening Array+ Kit (48 Spl)
20119540
Reagent kit for 48 samples. Contains 1 48-sample BeadChip and all required reagents for EX Methylation Assay after bisulfite conversion. Sample processing requires the iScan System and Infinium Automated Pipetting System with ILASS. View less
Inf Methyl Screening Array+ Kit (96 Spl)
20119541
Reagent kit for 96 samples. Contains 2 48-sample BeadChips and all required reagents for EX Methylation Assay after bisulfite conversion. Sample processing requires the iScan System and Infinium Automated Pipetting System with ILASS. View less
Inf Methyl Screening Array+ Kit (1152 Spl)
20119542
Reagent kit for 1,152 samples. Contains 24 48-sample BeadChips and all required reagents for EX Methylation Assay after bisulfite conversion. Sample processing requires the iScan System and Infinium Automated Pipetting System with ILASS. View less
Showing of
Product
Qty
Unit price
Remove
Total ( items)
You have not selected any items yet.
Please add these products to your cart before leaving the page to avoid losing them.
How is the Infinium Methylation Screening Array different from the Infinium MethylationEPIC v2.0?
The Infinium MethylationEPIC v2.0 BeadChip offers a comprehensive genome-wide discovery tool with ~930K CpG sites covering >99% RefSeq genes with promoter and enhancer-focused content. The Infinium MethylationEPIC v2.0 BeadChip is ideal for projects in which methylation profiling across all genes and promoter regions is desired for maximum discovery power. It is also ideal for all oncology applications due to its coverage of critical oncogenes, compatibility with FFPE tissue, and copy number variation profiling capabilities.
The Infinium Methylation Screening Array offers a more cost-effective methylation screening tool for the study of common disease for population health projects. The ~270K methylation sites are focused on known trait associations from epigenome-wide association studies over the past decade, as well as novel content covering cell-type specific methylation, intermediate methylation, CoRSIVs, SNPs, and more. The Infinium Methylation Screening Array is ideal for large-scale epigenome-wide association studies (EWAS) and biobank screening projects.
A comprehensive product comparison table can be found on the Infinium Methylation Screening Array datasheet.
Is the Infinium Methylation Screening Array workflow different from the Infinium MethylationEPIC v2.0 workflow?
Yes, the Methylation Screening Array requires the EX Methylation workflow while the EPICv2.0 uses HD Methylation workflow. EX Methylation delivers new sample processing efficiencies enabled by the 48 sample EX BeadChip and automation., and has a lower DNA input of 50ng. The EX Methylation workflow requires the Infinium Automated Pipetting System with ILASS for automated liquid handling.
Where can I find detailed trait annotations for the Infinium Methylation Screening Array?
While the Illumina product manifest file provides basic genomic mapping annotations, the Zhou Lab Infinium Annotations GitHub page provides detailed annotations including trait associations covered on the Infinium Methylation Screening Array.
Can the Infinium Methylation Screening Array be used for oncology research including cancer classification?
The Infinium Methylation Screening Array is not recommended for oncology applications due to the following reasons:
· FFPE compatibility unvalidated
· Does not have sufficient coverage of probes for widely-used cancer classifiers
· Does not cover MGMT promoter methylation
· Limited ability to profile copy number variations
· Larger minimum batch size of 48 samples per BeadChip may result in longer turnaround times for results
Which software packages can be used to analyze methylation controls for the EX Methylation assay?
Illumina offers user friendly tools to analyze Infinium controls built into the Methylation Screening Array. For more information visit the Methylation Array Data Analysis Tips page.
How long does it take to scan a MethylationEPIC v2.0 array on the iScan System?
The scan takes ~20 minutes per BeadChip.
Frequently purchased
Other products to consider
