Illumina, 20139070, TruSight FFPE QC Kit

Overview
DNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is degraded by the fixation process and can vary greatly in quality and usability for sequencing applications. The Illumina TruSight FFPE QC kit uses a real-time PCR assay targeting a 204 bp amplicon to evaluate the quality and suitability of prospective FFPE DNA samples for sequencing.

Key features
Evaluate sample quality accurately with standard real-time PCR methods
Perform up to 384 reactions per kit
Achieve clear results with a simple workflow
The Illumina TruSight FFPE QC Kit uses a real-time PCR assay to evaluate DNA sample quality. The assay is compatible with FFPE DNA extracted using commercially available kits from various suppliers for exceptional flexibility.
Each kit provides primers and reference DNA template for 384 reactions. The workflow will be familiar to labs with experience using common PCR methods, including standard real-time PCR instrumentation and reagents.
The workflow follows an easy real-time PCR protocol that can be performed with qPCR instruments and reagents from a range of manufacturers.

Applications
TruSight FFPE QC Kit uses a real-time PCR assay to evaluate the quality of prospective DNA samples, ensuring their suitability for downstream sequencing applications.

Related applications and methods
Cellular & Molecular Biology Research
Array and NGS technologies from Illumina enable analysis across the genome, transcriptome, and epigenome, broadening cellular research possibilities.

Resources
Sequencing FFPE Samples: Introduction
This webinar introduces FFPE sample types, extraction methods, and library prep options, offering guidance for both new and experienced users working with degraded or limited DNA.
TruSight FFPE QC Kit
20139070
TruSight™ FFPE QC Kit uses a real-time PCR assay to evaluate the quality of prospective DNA samples. View less
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