Description
Overview
Illumina miRNA Prep enables robust miRNA-specific library preparation while minimizing reaction biases and eliminating adapter dimers. Unique Molecular Identifiers (UMIs) tag each miRNA at an early stage, removing PCR and sequencing biases.
Key features
Improved workflow eliminates the need for gel purification, saving time and reducing sample loss
Effectively prevents adapter dimer formation with an enhanced library preparation, even from small amounts of RNA
Reduces biases and background interference while producing high-quality, miRNA-targeted libraries
Optimized for ultralow input levels, crucial for detecting circulating miRNA biomarkers
Incorporates UMIs for unbiased, accurate quantification of mature miRNAs using next-generation sequencing
Illumina miRNA Prep generates reliable, miRNA-specific libraries, minimizes reaction biases, and eliminates adapter dimers, even from a low input of total RNA.
No gel electrophoresis, excision, and elution steps are required, providing a substantial reduction in sample loss and improved time savings.
Illumina miRNA Prep enables high-throughput sequencing on Illumina MiSeq, NextSeq, and NovaSeq Sequencing Systems using up to 384 unique dual indexes.
Applications
Illumina miRNA Prep enables preparation of miRNA and small RNA sequencing libraries for a wide variety of sample types in 7 hours, with 3 hours of hands-on time, using as little as 1 ng of total RNA input.
Example workflow
Illumina miRNA Prep (96 samples)
NovaSeq X Series
NextSeq 1000 and NextSeq 2000 Sequencing Systems
MiSeq i100 Series
Illumina Connected Analytics
DRAGEN miRNA
Project recommendations
Instrument: Recommended number of samples
NextSeq 2000 System: P2 XLEAP-SBS (100 cycles) - 96 samples P3 XLEAP-SBS (100 cycles) - 192 samples P4 XLEAP-SBS (100 cycles) - 384 samples (recommended 5-10M reads/sample)
P2 XLEAP-SBS (100 cycles) - 96 samples
P3 XLEAP-SBS (100 cycles) - 192 samples
P4 XLEAP-SBS (100 cycles) - 384 samples
(recommended 5-10M reads/sample)
1 x 75 bp
MiSeq i100 System: 25M Reagent Kit (100 cycles) - 6 samples
25M Reagent Kit (100 cycles) - 6 samples
Related applications and methods
RNA sequencing
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA.
miRNA and small RNA sequencing
Isolate and sequence small RNA species, such as miRNAs and other small noncoding RNAs, or study differential expression of small RNAs.
Figures
Illumina miRNA Prep chemistry
Adapters are ligated to 5' and 3' ends to ensure preferential amplification of miRNAs. Unique molecular identifiers (UMIs) are integrated during the reverse transcription (RT) step, enabling digital quantification and correcting for amplification bias. A rapid, bead-based cleanup replaces gel purification, streamlining the workflow to just seven hours.
Illumina miRNA Prep and TruSeq Small RNA workflow comparison
By eliminating gel electrophoresis and purification, Illumina miPrep mRNA Prep delivers a faster workflow with reduced hands-on time compared to TruSeq Small RNA.
Unbiased miRNA quantification using UMIs
By incorporating unique molecular identifiers (UMIs) early in the workflow, most sequencing reads are mapped to unique miRNAs, minimizing bias from adapter dimers.
Robust miRNA detection in serum samples
Illumina miRNA Prep provides robust, reproducible miRNA detection in biofluids such as serum. (A) Detection of miRNA from replicate serum samples. (B) Reads of mapped miRNAs compared to adapter dimers from replicate serum samples.
Illumina miRNA Prep (96 samples)
20145030
Includes all reagents for library prep, indexes not included View less
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Illumina miRNA Indexes Set A (96 Indexes, 96 Samples)
20145031
96 unique dual indexes, Set A View less
Illumina miRNA Indexes Set B (96 Indexes, 96 Samples)
20145032
96 unique dual indexes, Set B View less
Illumina miRNA Indexes Set C (96 Indexes, 96 Samples)
20145033
96 unique dual indexes, Set C View less
Illumina miRNA Indexes Set D (96 Indexes, 96 Samples)
20145034
96 unique dual indexes, Set D View less
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What sample types are compatible with Illumina miRNA Prep?
Illumina miRNA Prep is compatible with total RNA isolated from cells, FFPE tissue, serum/plasma, and fresh/frozen tissue from virtually any species.
Does miRNA need to be enriched before using Illumina miRNA Prep?
No miRNA enrichment is required—Illumina miRNA Prep starts with total RNA.
Can I use other Illumina RNA UDI plates with Illumina miRNA Prep?
No, Illumina miRNA Prep is designed to work with Illumina miRNA UD Index Plates A-D and is not compatible with other Illumina RNA UDI plates.
Is Illumina miRNA Prep compatible with other types of small RNA beyond miRNA?
The kit is optimized to capture small RNAs in the 18-40 base range. Other small RNAs that are compatible with Illumina miRNA Prep include PIWA-interacting RNA (piRNA), small interfering RNA (siRNA), and transfer RNA-derived small RNA (tsRNA).
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