Illumina, FC-110-3001, PhiX Control v3

Name: PhiX Control v3
Brand: Illumina
SKU: FC-110-3001

Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 300 base pairs. (10ul of 10nM template solution)

Catalog Number: FC-110-3001

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Category

NGS Library Preparation Kits

TagsSequencing kits Sequencing reagents & flow cells

Description

Overview
MiSeq i100 Series Reagent Kits deliver flexible, efficient, and sustainable workflows.

Key features
5M-100M reads and 1.5 Gb to 30 Gb of highly accurate and reliable data per run enabling user to scale sample throughput and perform deeper sequencing
100-, 300-, 600-, and 1000-cycle kits to support a wide range of applications – including small whole genome sequencing, targeted gene sequencing, transcriptomics, and library QC
These kits offer streamlined, load-and go reagents and room temperature shipping and storage. This innovation removes the requirement for cold chain logistics and freezer storage, delivering remarkable benefits in sustainability and user experience. Increase sample turnaround time and reduce run inconsistencies by eliminating thawing.
The MiSeq i100 Series is powered by XLEAP-SBS chemistry, our fastest, most robust, and highest-quality sequencing by synthesis (SBS) chemistry. It provides significant improvements in stability, speed, and performance across Illumina kits, delivering higher confidence in generated data and expediting project completion.

How does the MiSeq i100 integrated cartridge simplify sequencing?
The MiSeq i100 Series Reagent Kits use integrated cartridges that include sequencing reagents and the flow cell, simplifying library loading and improving efficiency throughout the sequencing run helping minimize errors. Easy maintenance with cartridge-based fluidics minimizes cross-contamination between samples and runs.

How long does a run on the MiSeq i100 Series take?
The MiSeq i100 Series has multiple reagent configurations supporting a variety of applications and sample throughputs. Each configuration will have different run times. Run times range from ~4-24 hr.
See the MiSeq i100 Series specifications for more information.

How many reads does the MiSeq i100 Series generate per run?
The MiSeq i100 Series can generate up to 100M single reads and 200M paired-end reads, depending on the flow cell type.
See the MiSeq i100 Series specifications for more information.

How many flow cell types are offered for the MiSeq i100 Series?
The MiSeq i100 Series offers up to 11 configurations with four flow cell types. The 5M and 25M flow cells are available on the MiSeq i100 System; the 5M, 25M, 50M, and 100M flow cells are available on the MiSeq i100 Plus System.
See the MiSeq i100 Series specifications for more information.

What are the differences between the MiSeq i100 and MiSeq i100 Plus Systems?
The MiSeq i100 System is a lower cost instrument that runs 5M and 25M flow cells to support lower data output and sample throughputs. The MiSeq i100 Plus System accesses a wider range of throughputs with 5M, 25M, 50M, and 100M flow cells for more sequencing per sample and larger studies.
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Specifications

General

Instruments	MiSeq i100 Plus System , MiSeq i100 System
Nucleic acid type	DNA, RNA
Reagent type	Paired-end sequencing, Single-read sequencing
Technology	Sequencing

FAQs

Question: What concentration and volume are supplied?

Answer:PhiX Control v3 is supplied as 10 µL of a 10 nM template solution.

Question: What is PhiX used for?

Answer:It is used as an Illumina sequencing control for run validation, quality monitoring, and optional spike-in to experimental libraries.

Question: What read lengths are supported?

Answer:The control is compatible with single-end and paired-end reads up to 300 bases.

Application Note

PhiX Control v3 is a ready-to-use Illumina sequencing control library derived from the well-characterized bacteriophage PhiX genome. This configuration contains 10 µL of a 10 nM template solution. The library can be sequenced by itself for instrument validation or spiked into experimental libraries as an internal run control. Because the PhiX sequence and expected base composition are known, it supports monitoring of sequencing quality, cluster generation, phasing and prephasing calculations, and overall run performance. The balanced base composition can also improve base-calling behavior in low-diversity sequencing runs when an appropriate PhiX spike-in is used. PhiX Control v3 is compatible with Illumina single-end and paired-end sequencing workflows with reads up to 300 bases.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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