Description
Overview
The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic variants in myeloid malignancies.
The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in:
Acute myeloid leukemia (AML)
Myelodysplastic syndrome (MDS)
Myeloproliferative neoplasms (MPN)
Chronic myelogenous leukemia (CML)
Chronic myelomonocytic leukemia (CMML)
Juvenile myelomonocytic leukemia (JMML)
The TruSight Myeloid Sequencing Panel covers 15 full genes (exons only) and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test. The result is an accurate, cost-effective solution that enables researchers to profile liquid tumors. View the gene list in the "Figures" section.
TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller. Filtering and annotation can then be performed using variant analysis software.
Example workflow
TruSight Myeloid Sequencing Panel
MiniSeq System
MiSeq System
NextSeq 550 System
BaseSpace TruSeq Amplicon App
Variant analysis software
Project recommendations
Instrument: Recommended number of samples
MiniSeq System: Samples per run: mid output: 2-3, high output: 8 (based on 95% amplicons at > 500x coverage)
Samples per run: mid output: 2-3, high output: 8 (based on 95% amplicons at > 500x coverage)
2 × 150 bp (max recommended)
MiSeq System: 8 samples per run with v3 reagents (based on 95% amplicons at > 500x coverage)
8 samples per run with v3 reagents (based on 95% amplicons at > 500x coverage)
NextSeq 550 System: Samples per run: mid output: 48, high output: 96 (based on 95% amplicons at > 500x coverage)
Samples per run: mid output: 48, high output: 96 (based on 95% amplicons at > 500x coverage)
Related applications and methods
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
Amplicon sequencing
Ultra-deep sequencing of PCR amplicons enables analysis of specific genomic regions of interest. Learn more about amplicon sequencing and find comprehensive solutions.
Cancer sequencing methods
NGS-based cancer sequencing methods provide more information in less time compared to single-gene and array-based approaches.
TruSight Myeloid Sequencing Panel Gene List
ABL1: CEBPA
ASXL1: CSF3R
ATRX: CUX1
BCOR: DNMT3A
BCORL1: ETV6/TEL
BRAF: EZH2
CALR: FBXW7
CBL: FLT3
CBLB: GATA1
CBLC: GATA2
CDKN2A: GNAS
TruSight Myeloid Sequencing Panel (96 samples)
FC-130-1010
TruSeq Custom Amplicon-based library preparation reagents for 54 genes (either full genes or targeted regions) associated with myeloid malignancies. Includes library preparation reagents and TruSight Myeloid oligos sufficient for 96 samples. View less
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TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples)
FC-130-1003
Each TruSeq Custom Amplicon Index Kit includes 96 unique indexes for preparation of up to 384 samples. View less
TruSeq Custom Amplicon Filter Plate (1 plate)
FC-130-1006
Includes a single replacement filter plate for use with the TruSeq Custom Amplicon assay. View less
TruSeq Index Plate Fixture & Collar Kit (2 each)
FC-130-1007
Assists in correctly arranging index primers for PCR amplification step. Includes two (2) TruSeq Index Plate Fixtures and two (2) Plate Collars. View less
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