Illumina, FC-131-2004, Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

Overview
Nextera DNA Indexes enable multiplexing of high-quality sequencing libraries generated from the Nextera XT DNA Library Preparation Kit or Illumina DNA Prep. Available in two adapter kits, the Nextera XT Index Kit v2 and Nextera DNA CD Indexes suit a range of throughput next-generation sequencing (NGS) applications.
Provide high-throughput sequencing of up to 384 samples
Accommodate lower throughput sequencing application needs
Offer low-cost indexes for labs on a budget

Combinatorial dual indexes
Nextera DNA Indexes are combinatorial dual (CD) indexes and cannot eliminate index hopping artifacts during demultiplexing. CD indexes work best for applications that do not require high sensitivity and for multiplexing on sequencing systems that use nonpatterned flow cells, such as the MiniSeq, MiSeq, and NextSeq 550 Systems.
It is recommended to use Illumina DNA/RNA UD Indexes for multiplexing on sequencing platforms with patterned flow cells such as the NextSeq 2000 System, NovaSeq 6000 System, and NovaSeq X Series to reduce index hopping or read misassignment during demultiplexing. Nextera XT v2 indexes and Nextera Combinatorial Dual (CD) indexes cannot eliminate read misassignment due to index hopping artifacts.

Additional information
Find kits compatible with Nextera DNA indexes and learn more about how to minimize index hopping on your next sequencing run.
Nextera XT DNA Library Preparation Kit
Illumina DNA Prep
Minimizing index hopping

Where do I find adapter and index sequences?
The Illumina Adapter Sequences Document lists index adapter sequences and all other adapter sequences.

How can I learn more about sample multiplexing?
Visiit our multiplex sequencing page for more information.
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