Illumina, HDA-CH-3003-24, OncoReveal Solid Tumor Panel v2 24 Rxn

Pillar oncoReveal Solid Tumor v2 Panel
Robust DNA NGS assay interrogating 48 genes across multiple solid tumor cancer types.
Pillar oncoReveal Solid Tumor v2 Panel Data sheet PDF < 1 MB
Data sheet PDF < 1 MB
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Overview
The Pillar oncoReveal Solid Tumor v2 Panel is a robust 48-gene assay that simultaneously tests for key mutations present in DNA from solid tumors, including non-small cell lung cancer (NSCLC), colorectal, melanoma, endometrial, pancreatic, gastrointestinal stromal tumor (GIST), bladder, thyroid, and gliomas from formalin-fixed, paraffin-embedded (FFPE) samples. Additionally, genes with potential importance in immuno-oncology such as POLD1 and POLE are analyzed.

Key features
Access a rapid workflow to go from sample to report within 48 hours
Achieve variant detection as low as 1% VAF without UIDs even with limited DNA input or poor sample quality
Improve lab efficiency and reduce “no calls”
The panel is designed to detect single nucleotide variants (SNVs) and insertion/deletions (indels) in DNA isolated from FFPE tissue.
The assay uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.
The workflow allows labs to maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab.

Applications
The Pillar oncoReveal Solid Tumor v2 Panel supports NGS-based targeted sequencing of numerous gene regions of interest, for researchers looking to explore the sequences of germline and FFPE DNA from solid tumor samples.

Example workflow
Pillar oncoReveal Solid Tumor v2 Panel
MiSeq i100 Series
MiSeqDx Instrument (RUO mode)
NextSeq 550 System
NextSeq 550Dx Instrument (RUO mode)
Illumina Connected Insights

Related applications and methods
Cancer genomics
Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.
Cancer sequencing methods
NGS-based cancer sequencing methods provide more information in less time compared to single-gene and array-based approaches.
Immuno-oncology research
Next-generation sequencing enables immuno-oncology researchers to gain insights into immunotherapy response factors and tumor immune evasion mechanisms.

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Gene List
Pillar oncoReveal Solid Tumor v2 Panel includes 48 genes.
Panel Specifications
Pillar oncoReveal Solid Tumor v2 Panel specifications for interrogation DNA isolated from FFPE tissue.
OncoReveal Solid Tumor Panel v2 24 Rxn
HDA-CH-3003-24
48 gene (24 sample) solid tumor DNA panel on SLIMamp™ workflow View less
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