| Assay time | < 8 hours |
|---|---|
| Hands-on time | ~3 hr |
| Input quantity | 20–60 ng DNA |
| Cancer type | Hematologic |
| Content specifications | 84 genes |
| Description | This NGS assay analyzes 84 genes relevant to lymphoid cancers with full coverage of 29 genes and hotspots in an additional 55 genes. |
| Instruments | MiSeq System , MiSeqDx in Research Mode , NextSeq 550Dx in Research Mode , MiSeq i100 System , MiSeq i100 Plus System |
| Mechanism of action | Multiplex PCR using tiled amplicon |
| Method | Targeted DNA sequencing , Amplicon sequencing |
| Nucleic acid type | DNA |
| Number of amplicons | 1 pool, 1046 amplicons |
| Number of reactions | 24 reactions per kit |
| On-target reads | 83.2% |
| Specialized sample types | Blood, Low-input samples, Bone marrow |
| Species category | Human |
| Target insert size | 213 bp |
| Technology | Sequencing |
| Uniformity | 85.9% |
| Variant class | Single nucleotide variants (SNVs), Insertions-deletions (indels) |
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