Illumina, PR-40-415101-00, SurePlex DNA Amplification System (50 reactions)

Name: SurePlex DNA Amplification System (50 reactions)
Brand: Illumina
SKU: PR-40-415101-00

Buffers and reagents for 50 reactions to amplify total DNA from single cells. Intended for use with 24sure assay or VeriSeq PGS applications.

Catalog Number: PR-40-415101-00

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Category

Single Cell Sequencing Reagents

Pack Size50 reactions

TagsClinical research products

Description

Overview
Whole-genome amplification technology can be used to increase the quantity of DNA in a single cell or a few cells. This amplification ensures sufficient amounts of DNA for next-generation sequencing of preimplantation genetic screening (PGS) samples.
The SurePlex DNA Amplification System reproducibly amplifies total DNA from single cells or a few cells of an embryo biopsy. It follows an easy-to-use single tube protocol to produce 2-5 μg of amplified DNA in 2 hours. Following SurePlex DNA amplification, samples are ready for use in research applications such as VeriSeq PGS (available from Vitrolife ).

Related applications and methods
Preimplantation genetic screening research
Preimplantation genetic screening (PGS), also known as PGT-A, determines the chromosomal status of an embryo by screening all 23 chromosome pairs.
Karyomapping research
Karyomapping techniques can assess the likelihood of an embryo carrying a gene mutation that may be linked to an inherited genetic disorder.
Cytogenomics
Together, next-generation sequencing and arrays enable cytogenomic analysis of chromosome aberrations for constitutional and cancer research.

Specifications

General

Assay time	~3 hours
Hands-on time	~1 hr
Input quantity	Single cell or multi-cell samples
Method	Targeted DNA sequencing
Nucleic acid type	DNA
Specialized sample types	Single cells
Species category	Human
Technology	Sequencing
Variant class	Chromosomal abnormalities, Structural variants, Copy number variants (CNVs)

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