Illumina, RS-122-2002, TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)

Overview
Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.

Multiplex capabilities
Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.

Minimal hands-on steps
Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.

Fast, automation-friendly workflow
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners .

Example workflow
TruSeq RNA Library Prep Kit v2
NextSeq 550 System
DRAGEN RNA
RNA-Seq Differential Expression
BaseSpace RNA Express App

Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: RNA profiling: 13–40 samples per run (based on 10 million reads per sample) Transcriptome analysis: 5–16 samples per run (based on 25 million reads per sample)
RNA profiling: 13–40 samples per run (based on 10 million reads per sample) Transcriptome analysis: 5–16 samples per run (based on 25 million reads per sample)
2 x 75 bp

Related applications and methods
RNA sequencing
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA.
mRNA sequencing
mRNA sequencing (mRNA-Seq) using NGS can identify both known and novel transcripts, and measure transcript abundance.
Gene expression analysis
Learn how to profile gene expression changes for a deeper understanding of biology.

TruSeq RNA Library Preparation Reagents provide significant savings in time and effort
Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.
TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)
RS-122-2001
Includes reagents and 12 of 24 indexes (Indexes: 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, 19) for preparing 48 total RNA samples. View less
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TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)
RS-122-2002
Includes reagents and 12 of 24 indexes (Indexes: 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27) for preparing 48 total RNA samples. View less
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