TruSight RNA Fusion Panel
Comprehensive gene fusion detection covering 507 genes from FFPE and other cancer research samples.
TruSight RNA Fusion Panel Data sheet PDF 1 MB
Data sheet PDF 1 MB
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Overview
The TruSight RNA Fusion Panel covers 507 fusion-associated genes related to cancer in a single NGS assay for research use, providing results from RNA in four days.
Industry-guided content for comprehensive detection of common and novel fusions
Optimized RNA sequencing for all sample types, including formalin-fixed, paraffin-embedded (FFPE) tissue
On-instrument software that displays fusion calls
A comprehensive view of cancer-related fusion genes
The TruSight RNA Fusion Panel covers hundreds of genes in a single assay, increasing the possibility of fusion detection in the first round of testing.
Simple, streamlined workflow
A simple integrated workflow includes library prep, sequencing, and data analysis and can be scaled according to the number of samples. RNA samples can be multiplexed and sequenced on a benchtop system to maximize lab budgets.
On-instrument software
On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman Database), and evidence of fusion-supporting reads.
Applications
TruSight RNA Fusion Panel enables gene fusion detection studies with known and novel partners in 507 fusion-associated genes in multiple cancer types using blood, bone marrow, or FFPE samples.
Example workflow
MiniSeq System
MiSeq System
NextSeq 550 System
Local Run Manager (RNA Fusion Module)
BaseSpace RNA-Seq Alignment App
Project recommendations
Instrument: Recommended number of samples
MiniSeq System: Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample)
Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample)
2 × 76 bp (max recommended)
MiSeq System: 8 samples per run with v3 reagents (based on 3 million reads per sample)
8 samples per run with v3 reagents (based on 3 million reads per sample)
NextSeq 550 System: 24 samples per run (mid output; based on 3 million reads per sample)
24 samples per run (mid output; based on 3 million reads per sample)
Related applications and methods
Cytogenomics
Together, next-generation sequencing and arrays enable cytogenomic analysis of chromosome aberrations for constitutional and cancer research.
Low-quality/FFPE RNA-Seq solutions
Find FFPE RNA sequencing solutions that yield high-quality results from degraded FFPE tissues and other low-quality samples.
Cancer RNA sequencing
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
TruSight RNA Fusion capture chemistry
The TruSight RNA Fusion Panel provides a simple, streamlined method for isolating targeted regions of interest from total RNA.
Fusion detection in FFPE samples using the TruSight RNA Fusion Panel
The Trusight RNA Fusion Panel is optimized for high performance from both high- and low-quality RNA sample types. Library prep requires as little as 10 ng total RNA, or 20 ng FFPE RNA. This low input requirement makes this panel ideal for reliable targeted analysis of limited quantity samples.
TruSight RNA Fusion Panel Set A (48 samples)
RS-304-1002
Kitted reagents for RNA library prep and enrichment of 48 total RNA samples (including FFPE) for sequencing. Each kit contains 12 unique indexes; 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, and 19. View less
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TruSight RNA Fusion Panel Set B (48 samples)
RS-304-1003
Kitted reagents for RNA library prep and enrichment of 48 total RNA samples (including FFPE) for sequencing. Each kit contains 12 unique indexes; 1, 3, 8, 9,10, 11, 20, 21, 22, 23, 25, and 27. View less
Trusight RNA Fusion Oligo Panel
20046101
Enrichment oligos only. Library prep and index adapter reagents need to be ordered separately View less
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What are high- and low-confidence fusion calls?
A high-confidence fusion call means that a fusion meets the threshold filters based on scores from calculated values of split read scores, paired read scores, break-end homology, and other features.
A low- confidence fusion call means that a fusion is listed as recurrent in the Mitelman database but does not pass or meet the minimum threshold score.
Learn more in Local Run Manager RNA Fusion Analysis Module Workflow Guide .
What does the Fusion Score mean?
The Fusion Score reflects the confidence in the fusion call, where 0 is low and 1 is high. Scores > 0.6 are reported as high- confidence fusion calls.
Can I automate the TruSight RNA Fusion Panel?
This assay is being automated by some of our automation providers . If you are interested in automating this workflow, contact your account manager.
Will the TruSight RNA Fusion Panel detect chromosomal translocations that do not generate a fusion gene?
Chromosomal translocations resulting in overexpression or deletion of a transcript can be reflected in gene expression levels but would not create a fusion gene. The Local Run Manager RNA Fusion module is not designed for detection of gene expression changes. To detect these changes, the RNA-Seq Alignment App in BaseSpace Sequence Hub is recommended. In addition, it is recommended to confirm these findings in DNA.
