Description
Overview
PhiX Controls are a reliable, adapter-ligated library used as a control for Illumina sequencing runs and derived from the small, well-characterized PhiX genome. They offer benefits for cluster generation, sequencing and alignment, and calibration for crosstalk matrix generation, phasing, and prephasing.
PhiX Controls can be rapidly aligned to estimate relevant sequencing by synthesis (SBS) metrics such as phasing and error rate. The ready-to-use library can add value to your workflow and increase confidence in your results.
PhiX Indexed Control (1000 cycles) has a longer insert required for longer read 2×500 sequencing applications. Use for sample demultiplexing and/or to provide additional diversity and color balance in index reads when spiked into low-plexity library pools.
Depending on the application, PhiX Controls may also be used:
As a high-concentration spike-in for unbalanced samples (genomes with AT or GC content of less than 40% or greater than 60%)
For troubleshooting cluster generation problems, to help determine whether an error is related to library preparation
As a low-concentration spike-in for alignment calculations and quantification efficiency
As a dedicated control lane alongside low-diversity samples
PhiX v3 is also available as an Illumina Advantage (TG) product.
Resources
What is PhiX Control v3 and what is its function in Illumina sequencing?
Learn more about the PhiX Control v3 library and how it's used as a run quality monitor, calibration control, and more.
PhiX loading concentrations for Illumina sequencing validation runs
See what the optimal loading concentrations and cluster densities are for each Illumina sequencing platform.
PhiX Indexed Control (1000 cycles)
20151542
Kitted Indexed DNA control for Illumina sequencing platforms. Compatible with paired End reads up to 500 base pairs. (10ul of 10nM template solution). View less
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PhiX Control v3
FC-110-3001
Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 300 base pairs. (10ul of 10nM template solution) View less
TG PhiX Control Kit v3
TG-110-3001
PhiX Control Kit v3 View less
NextSeq PhiX Control Kit
FC-110-3002
DNA control for the NextSeq Illumina sequencing platform. Compatible with single and paired-end reads up to 300 bp. 10 µl of a 10 nM PhiX solution. View less
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Do the PhiX Index Control (1000 cycles) index combinations overlap?
The index combinations do not overlap with any current Illumina or IDT index combinations.
How do I determine the index sequences for PhiX Index Control (1000 cycles)?
Refer to the PhiX Indexed Control (1000 Cycle) Product Information for the index sequences.
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