Leica, AD-465-35, ATTO 465 NHS-ester Fluorescent Label 5 mg

Optical properties
λ abs = 453 nm
ε max = 7.5×10 4 M -1 cm -1
λ fl = 506 nm
n fl = 70 %
τ fl = 5.0 ns
CF 260 = ε 260 /ε max = 1.09
CF 280 = ε 280 / ε max = 0.48
ATTO 465 is a novel fluorescent label derived from the well-known dye Acriflavin. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. In aqueous solution the dye shows a surprisingly large Stokes-shift of 55 nm. The dye shows intense and long-lived phosphorescence in solid matrix, in particular at low temperatures. ATTO 465 is moderately hydrophilic. The fluorescence can be excited efficiently in the range 420 - 465 nm .