Leica, AD-488-75, ATTO 488 Biotin Fluorescent Label 5 mg

Optical properties
λ abs = 500 nm
ε max = 9.0×10 4 M -1 cm -1
λ fl = 520 nm
n fl = 80 %
τ fl = 4.1 ns
CF 260 = ε 260 /ε max = 0.22
CF 280 = ε 280 / ε max = 0.09
ATTO 488 is a hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield, and exceptional thermal and photo-stability. Thus ATTO 488 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS) , fluorescence in-situ hybridization (FISH and many more. The fluorescence is excited most efficiently in the range 480 - 515 nm .
A suitable source of excitation is the 488 nm line of an Argon-Ion laser.