Leica, AD-514-75, ATTO 514 Biotin Fluorescent Label 5 mg

Optical properties
λ abs = 511 nm
ε max = 1.15×10 5 M -1 cm -1
λ fl = 532 nm
n fl = 85 %
τ fl = 3.9 ns
CF 260 = ε 260 /ε max = 0.21
CF 280 = ε 280 / ε max = 0.07
ATTO 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield, and exceptional thermal and photo-stability. Thus ATTO 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS) , fluorescence in-situ hybridization (FISH and many more.
The fluorescence is excited most efficiently in the range 510 - 535 nm . A suitable source of excitation is the 514 nm line of an Argon-Ion laser.