Leica, AD-532-25, ATTO 532 Carboxy Fluorescent Label 5 mg

Optical properties
λ abs = 532 nm
ε max = 1.15×10 5 M -1 cm -1
λ fl = 552 nm
n fl = 90 %
τ fl = 3.8 ns
CF 260 = ε 260 /ε max = 0.20
CF 280 = ε 280 / ε max = 0.09
ATTO 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photo-stability, and excellent water solubility. Thus ATTO 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS) , fluorescence in-situ hybridization (FISH and many more.
The fluorescence is excited most efficiently in the range 515 - 545 nm . A suitable excitation source for ATTO 532 is the 532 nm output of the frequency-doubled Nd:YAG laser.