Leica, AD-542-75, ATTO 542 Biotin Fluorescent Label 5 mg

Optical properties
λ abs = 542 nm
ε max = 1.2×10 5 M -1 cm -1
λ fl = 562 nm
n fl = 93 %
τ fl = 3.7 ns
CF 260 = ε 260 /ε max = 0.18
CF 280 = ε 280 / ε max = 0.08
ATTO 542 is a new fluorescent label related to ATTO 532 .
The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy ( PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS) , fluorescence in-situ hybridization (FISH and many more.
After coupling to a substrate the dye carries a net electrical charge of - 3. The fluorescence is excited most efficiently in the range 520 - 555 nm .