Leica, AD-565-125, ATTO 565 Hydrazide Fluorescent Label 5 mg

Optical properties
λ abs = 564 nm
ε max = 1.2×10 5 M -1 cm -1
λ fl = 590 nm
n fl = 90 %
τ fl = 4.0 ns
CF 260 = ε 260 /ε max = 0.27
CF 280 = ε 280 / ε max = 0.12
ATTO 565 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 565 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS) , fluorescence in-situ hybridization (FISH and many more.
The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 545 - 575 nm . As supplied ATTO 565 consists of a mixture of two isomers with practically identical absorption and fluorescence. However, on request the dye can also be supplied as single isomer.