Description
Optical properties
λ abs = 603 nm
ε max = 1.2×10 5 M -1 cm -1
λ fl = 626 nm
n fl = 85 %
τ fl = 3.9 ns
CF 260 = ε 260 /ε max = 0.22
CF 280 = ε 280 / ε max = 0.50
ATTO 594 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. Thus ATTO 594 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS) , fluorescence in-situ hybridization (FISH and many more.
ATTO 594 is very hydrophilic and shows a very good water solubility. After coupling to a substrate the label carries a net electrical charge of -1. The fluorescence is excited most efficiently in the range 580 - 615 nm.